A Recombinant HAV Expressing a Neutralization Epitope of HEV Induces Immune Response against HAV and HEV in Mice

Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal-oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector f...

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Bibliographic Details
Published in:Viruses 2017-09, Vol.9 (9), p.260
Main Authors: Xiang, Kui, Kusov, Yuri, Ying, Guan, Yan, Wang, Shan, Yi, Jinyuan, Wu, Na, Yin, Yan, Zhou, Hongjun, Li, Maosheng, Sun
Format: Article
Language:English
Subjects:
Animals
Capsid protein
Capsid Proteins - genetics
Capsid Proteins - immunology
combined vaccine
Combined vaccines
Developing countries
Disease transmission
Epitopes
Epitopes - immunology
Genetic Vectors
Hepatitis
Hepatitis A
Hepatitis A - immunology
Hepatitis A - virology
Hepatitis A virus - genetics
Hepatitis A virus - immunology
Hepatitis Antibodies - biosynthesis
Hepatitis Antibodies - immunology
hepatitis E
Hepatitis E - immunology
Hepatitis E - virology
Hepatitis E virus - genetics
Hepatitis E virus - immunology
Immune response
Immunization
LDCs
Mice
neutralization epitope
Neutralization Tests
Public health
Vaccination
Vaccines
Vaccines, Combined - administration & dosage
Vaccines, Combined - genetics
Vaccines, Combined - immunology
vector
Viral Hepatitis Vaccines - administration & dosage
Viral Hepatitis Vaccines - genetics
Viral Hepatitis Vaccines - immunology
virus
Viruses
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Summary:Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal-oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector for the development of a combination vaccine against hepatitis A and E infections, recombinant HAV-HEp148 was created as a vector to express an HEV neutralization epitope (HEp148) located at aa 459-606 of the HEV capsid protein. The recombinant virus expressed the HEp148 protein in a partially dimerized state in HAV-susceptible cells. Immunization with the HAV-HEp148 virus induced a strong HAV- and HEV-specific immune response in mice. Thus, the present study demonstrates a novel approach to the development of a combined hepatitis A and E vaccine.
ISSN:1999-4915
1999-4915
DOI:10.3390/v9090260