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A Recombinant HAV Expressing a Neutralization Epitope of HEV Induces Immune Response against HAV and HEV in Mice
Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal-oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector f...
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| Published in: | Viruses 2017-09, Vol.9 (9), p.260 |
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| Main Authors: | , , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c466t-4d3717cd40ffc804be582324ec744dbc8ed87ddb47ef1a5ce2156f6d898698d53 |
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| container_issue | 9 |
| container_start_page | 260 |
| container_title | Viruses |
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| creator | Xiang, Kui Kusov, Yuri Ying, Guan Yan, Wang Shan, Yi Jinyuan, Wu Na, Yin Yan, Zhou Hongjun, Li Maosheng, Sun |
| description | Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal-oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector for the development of a combination vaccine against hepatitis A and E infections, recombinant HAV-HEp148 was created as a vector to express an HEV neutralization epitope (HEp148) located at aa 459-606 of the HEV capsid protein. The recombinant virus expressed the HEp148 protein in a partially dimerized state in HAV-susceptible cells. Immunization with the HAV-HEp148 virus induced a strong HAV- and HEV-specific immune response in mice. Thus, the present study demonstrates a novel approach to the development of a combined hepatitis A and E vaccine. |
| doi_str_mv | 10.3390/v9090260 |
| format | article |
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Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector for the development of a combination vaccine against hepatitis A and E infections, recombinant HAV-HEp148 was created as a vector to express an HEV neutralization epitope (HEp148) located at aa 459-606 of the HEV capsid protein. The recombinant virus expressed the HEp148 protein in a partially dimerized state in HAV-susceptible cells. Immunization with the HAV-HEp148 virus induced a strong HAV- and HEV-specific immune response in mice. Thus, the present study demonstrates a novel approach to the development of a combined hepatitis A and E vaccine.</description><identifier>ISSN: 1999-4915</identifier><identifier>EISSN: 1999-4915</identifier><identifier>DOI: 10.3390/v9090260</identifier><identifier>PMID: 28914805</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Animals ; Capsid protein ; Capsid Proteins - genetics ; Capsid Proteins - immunology ; combined vaccine ; Combined vaccines ; Developing countries ; Disease transmission ; Epitopes ; Epitopes - immunology ; Genetic Vectors ; Hepatitis ; Hepatitis A ; Hepatitis A - immunology ; Hepatitis A - virology ; Hepatitis A virus - genetics ; Hepatitis A virus - immunology ; Hepatitis Antibodies - biosynthesis ; Hepatitis Antibodies - immunology ; hepatitis E ; Hepatitis E - immunology ; Hepatitis E - virology ; Hepatitis E virus - genetics ; Hepatitis E virus - immunology ; Immune response ; Immunization ; LDCs ; Mice ; neutralization epitope ; Neutralization Tests ; Public health ; Vaccination ; Vaccines ; Vaccines, Combined - administration & dosage ; Vaccines, Combined - genetics ; Vaccines, Combined - immunology ; vector ; Viral Hepatitis Vaccines - administration & dosage ; Viral Hepatitis Vaccines - genetics ; Viral Hepatitis Vaccines - immunology ; virus ; Viruses</subject><ispartof>Viruses, 2017-09, Vol.9 (9), p.260</ispartof><rights>Copyright MDPI AG 2017</rights><rights>2017 by the authors. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-4d3717cd40ffc804be582324ec744dbc8ed87ddb47ef1a5ce2156f6d898698d53</citedby><cites>FETCH-LOGICAL-c466t-4d3717cd40ffc804be582324ec744dbc8ed87ddb47ef1a5ce2156f6d898698d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1952045017/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1952045017?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28914805$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiang, Kui</creatorcontrib><creatorcontrib>Kusov, Yuri</creatorcontrib><creatorcontrib>Ying, Guan</creatorcontrib><creatorcontrib>Yan, Wang</creatorcontrib><creatorcontrib>Shan, Yi</creatorcontrib><creatorcontrib>Jinyuan, Wu</creatorcontrib><creatorcontrib>Na, Yin</creatorcontrib><creatorcontrib>Yan, Zhou</creatorcontrib><creatorcontrib>Hongjun, Li</creatorcontrib><creatorcontrib>Maosheng, Sun</creatorcontrib><title>A Recombinant HAV Expressing a Neutralization Epitope of HEV Induces Immune Response against HAV and HEV in Mice</title><title>Viruses</title><addtitle>Viruses</addtitle><description>Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal-oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector for the development of a combination vaccine against hepatitis A and E infections, recombinant HAV-HEp148 was created as a vector to express an HEV neutralization epitope (HEp148) located at aa 459-606 of the HEV capsid protein. The recombinant virus expressed the HEp148 protein in a partially dimerized state in HAV-susceptible cells. Immunization with the HAV-HEp148 virus induced a strong HAV- and HEV-specific immune response in mice. Thus, the present study demonstrates a novel approach to the development of a combined hepatitis A and E vaccine.</description><subject>Animals</subject><subject>Capsid protein</subject><subject>Capsid Proteins - genetics</subject><subject>Capsid Proteins - immunology</subject><subject>combined vaccine</subject><subject>Combined vaccines</subject><subject>Developing countries</subject><subject>Disease transmission</subject><subject>Epitopes</subject><subject>Epitopes - immunology</subject><subject>Genetic Vectors</subject><subject>Hepatitis</subject><subject>Hepatitis A</subject><subject>Hepatitis A - immunology</subject><subject>Hepatitis A - virology</subject><subject>Hepatitis A virus - genetics</subject><subject>Hepatitis A virus - immunology</subject><subject>Hepatitis Antibodies - biosynthesis</subject><subject>Hepatitis Antibodies - immunology</subject><subject>hepatitis E</subject><subject>Hepatitis E - immunology</subject><subject>Hepatitis E - virology</subject><subject>Hepatitis E virus - genetics</subject><subject>Hepatitis E virus - immunology</subject><subject>Immune response</subject><subject>Immunization</subject><subject>LDCs</subject><subject>Mice</subject><subject>neutralization epitope</subject><subject>Neutralization Tests</subject><subject>Public health</subject><subject>Vaccination</subject><subject>Vaccines</subject><subject>Vaccines, Combined - administration & dosage</subject><subject>Vaccines, Combined - genetics</subject><subject>Vaccines, Combined - immunology</subject><subject>vector</subject><subject>Viral Hepatitis Vaccines - administration & dosage</subject><subject>Viral Hepatitis Vaccines - genetics</subject><subject>Viral Hepatitis Vaccines - immunology</subject><subject>virus</subject><subject>Viruses</subject><issn>1999-4915</issn><issn>1999-4915</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkl1rFDEUhgex2FoFf4EEvPFmazKTzxthKau7UFsQ7W3IJGfWLDPJmMwU9debdvt9lZA8eTi8eavqHcEnTaPwpyuFFa45flEdEaXUgirCXj7aH1avc95hzLnC4lV1WEtFqMTsqBqX6DvYOLQ-mDCh9fISrf6MCXL2YYsMOod5Sqb3_8zkY0Cr0U9xBBQ7tF5dok1ws4WMNsMwByimPMaQAZmt8SHvdSa4G9YH9M1beFMddKbP8PZ2Pa5-fln9OF0vzi6-bk6XZwtLOZ8W1DWCCOso7jorMW2BybqpKVhBqWutBCeFcy0V0BHDLNSE8Y47qSRX0rHmuNrsvS6anR6TH0z6q6Px-uYgpq02afK2B21teVOTjglmaC1ANg4LU_IRWBDJ2-L6vHeNczuAsxCuM3kifXoT_C-9jVeacSLLvxTBx1tBir9nyJMefLbQ9yZAnLMmimLMOG1oQT88Q3dxTqFEVShWY8owEQ9Cm2LOCbr7YQjW15XQd5Uo6PvHw9-Ddx1o_gOHra9V</recordid><startdate>20170915</startdate><enddate>20170915</enddate><creator>Xiang, Kui</creator><creator>Kusov, Yuri</creator><creator>Ying, Guan</creator><creator>Yan, Wang</creator><creator>Shan, Yi</creator><creator>Jinyuan, Wu</creator><creator>Na, Yin</creator><creator>Yan, Zhou</creator><creator>Hongjun, Li</creator><creator>Maosheng, Sun</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20170915</creationdate><title>A Recombinant HAV Expressing a Neutralization Epitope of HEV Induces Immune Response against HAV and HEV in Mice</title><author>Xiang, Kui ; Kusov, Yuri ; Ying, Guan ; Yan, Wang ; Shan, Yi ; Jinyuan, Wu ; Na, Yin ; Yan, Zhou ; Hongjun, Li ; Maosheng, Sun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-4d3717cd40ffc804be582324ec744dbc8ed87ddb47ef1a5ce2156f6d898698d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Capsid protein</topic><topic>Capsid Proteins - 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| identifier | ISSN: 1999-4915 |
| ispartof | Viruses, 2017-09, Vol.9 (9), p.260 |
| issn | 1999-4915 1999-4915 |
| language | eng |
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| source | Publicly Available Content (ProQuest); PubMed Central |
| subjects | Animals Capsid protein Capsid Proteins - genetics Capsid Proteins - immunology combined vaccine Combined vaccines Developing countries Disease transmission Epitopes Epitopes - immunology Genetic Vectors Hepatitis Hepatitis A Hepatitis A - immunology Hepatitis A - virology Hepatitis A virus - genetics Hepatitis A virus - immunology Hepatitis Antibodies - biosynthesis Hepatitis Antibodies - immunology hepatitis E Hepatitis E - immunology Hepatitis E - virology Hepatitis E virus - genetics Hepatitis E virus - immunology Immune response Immunization LDCs Mice neutralization epitope Neutralization Tests Public health Vaccination Vaccines Vaccines, Combined - administration & dosage Vaccines, Combined - genetics Vaccines, Combined - immunology vector Viral Hepatitis Vaccines - administration & dosage Viral Hepatitis Vaccines - genetics Viral Hepatitis Vaccines - immunology virus Viruses |
| title | A Recombinant HAV Expressing a Neutralization Epitope of HEV Induces Immune Response against HAV and HEV in Mice |
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