Identification and expression analyses of B3 genes reveal lineage-specific evolution and potential roles of REM genes in pepper

• Published in: BMC plant biology 2024-03, Vol.24 (1), p.201-13, Article 201
• Main Authors: Park, Young-Soo, Cho, Hye Jeong, Kim, Seungill
• Format: Article
• Language: English
• Subjects: Amino acids; Annotations; Arabidopsis - genetics; Arabidopsis thaliana; Bacterial infections; Biological evolution; Botanical research; Capsicum - metabolism; Capsicum annuum; Chromosomes; DNA binding proteins; Evolution; Evolutionary genetics; Gene expression; Gene Expression Regulation, Plant; Genes; Genes, Plant - genetics; Genetic aspects; Genetic transcription; Genomes; Genomics; Hormones; Mannitol; Multigene Family; Pepper; Peppers; Phylogenetics; Phylogeny; Plant growth; Plant Proteins - metabolism; Plants; Re-annotation; REM; Solanaceae; Speciation; Subgroups; Transcription; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Vegetables
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-04897-w

The B3 gene family, one of the largest plant-specific transcription factors, plays important roles in plant growth, seed development, and hormones. However, the B3 gene family, especially the REM subfamily, has not been systematically and functionally studied. In this study, we performed genome-wide re-annotation of B3 genes in five Solanaceae plants, Arabidopsis thaliana, and Oryza sativa, and finally predicted 1,039 B3 genes, including 231 (22.2%) newly annotated genes. We found a striking abundance of REM genes in pepper species (Capsicum annuum, Capsicum baccatum, and Capsicum chinense). Comparative motif analysis revealed that REM and other subfamilies (ABI3/VP1, ARF, RAV, and HSI) consist of different amino acids. We verified that the large number of REM genes in pepper were included in the specific subgroup (G8) through the phylogenetic analysis. Chromosome location and evolutionary analyses suggested that the G8 subgroup genes evolved mainly via a pepper-specific recent tandem duplication on chromosomes 1 and 3 after speciation between pepper and other Solanaceae. RNA-seq analyses suggested the potential functions of REM genes under salt, heat, cold, and mannitol stress conditions in pepper (C. annuum). Our study provides evolutionary and functional insights into the REM gene family in pepper.