Growth factors profile in conditioned medium human adipose tissue-derived mesenchymal stem cells (CM-hATMSCs)

• Published in: Clinical Nutrition Experimental 2019-04, Vol.24, p.34-44
• Main Authors: Noverina, Rachmawati, Widowati, Wahyu, Ayuningtyas, Wireni, Kurniawan, Dedy, Afifah, Ervi, Laksmitawati, Dian Ratih, Rinendyaputri, Ratih, Rilianawati, Rilianawati, Faried, Ahmad, Bachtiar, Indra, Wirakusumah, Firman Fuad
• Format: Article
• Language: English
• Subjects: Conditioned medium; Fresh frozen plasma; Growth factor; Human adipose tissue derived-mesenchymal stem cells; Wound healing
• ISSN: 2352-9393; 2352-9393
• DOI: 10.1016/j.yclnex.2019.01.002

Stem cell-derived by conditioned medium has potential as regenerative agent of skin cells because it contains many growth factors, cytokines and other regenerative biomolecules. Conditioned medium (CM) from human adipose tissue-derived MSCs (CM-hATMSCs) also has been known enriched growth factors that play an important role in epithelial wound repair, reduce wrinkles, and enhance wound healing. This study was performed to evaluate growth factors in CM such as Transforming Growth Factor (TGF)-β1, TGF-β2, Vascular Endothelial Growth Factor (VEGF)-2, Vascular Cell Adhesion Molecule (VCAM) 1, Epidermal Growth Factor (EGF), Fibroblast Growth Factor (FGF) in CM-hATMSCs treated with Fresh Frozen Plasma (FFP) and non-FFP at various passages. This study used ELISA method to measure the growth factors in CM-hATMSCs. FGF was the highest modulator among FFP and non-FFP-treated cells at many passages compared to the others growth factor. Both FFP and non-FFP-treated cells showed significant difference (P