Presence of Protease Inhibitor 9 and Granzyme B in Healthy and Pathological Human Corneas

The aim of this study was to find out whether protease inhibitor 9 ( ) and granzyme B ( ) molecules that contribute to immune response and the immunological privilege of various tissues are expressed in healthy and pathological human corneas. Using cryosections, cell imprints of control corneosclera...

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Bibliographic Details
Published in:Biology (Basel, Switzerland) Switzerland), 2022-05, Vol.11 (5), p.793
Main Authors: Reinstein Merjava, Stanislava, Kossl, Jan, Neuwirth, Ales, Skalicka, Pavlina, Hlinomazova, Zuzana, Holan, Vladimir, Jirsova, Katerina
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Apoptosis
CD45 antigen
Cloning
Conjunctiva
Cornea
corneal endothelium
corneal epithelium
Corneal transplantation
Cytokines
Cytotoxicity
Endothelium
Epithelium
Explants
Eye diseases
Gene expression
Graft rejection
Granzyme B
Herpes viruses
Histocompatibility antigen HLA
Immune response
Immunohistochemistry
Inflammation
Keratitis
Laboratories
Localization
Lymphocytes
Melting
protease inhibitor 9
Proteinase inhibitors
Proteins
Stroma
Transplants & implants
Tumor necrosis factor-TNF
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Summary:The aim of this study was to find out whether protease inhibitor 9 ( ) and granzyme B ( ) molecules that contribute to immune response and the immunological privilege of various tissues are expressed in healthy and pathological human corneas. Using cryosections, cell imprints of control corneoscleral discs, we showed that was expressed particularly in the endothelium, the superficial and suprabasal epithelium of healthy corneas, limbus, and conjunctiva. was localized in healthy corneal and conjunctival epithelium, while the endothelium showed weak immunostaining. The expression of PI-6 and was confirmed by qRT-PCR. Increased expression levels of the and genes were determined when the corneas were cultured with proinflammatory cytokines. Fluorescent and enzymatic immunohistochemistry of pathological corneal explants (corneal melting and herpes virus keratitis) showed pronounced , , human leucocyte antigen (HLA)-DR, and leukocyte-common antigen (CD45) signals localized in multicellular stromal infiltrates and inflammatory cells scattered in the corneal stroma. We conclude that increased expression of the and proteins under pathological conditions and their upregulation in an inflammatory environment indicate their participation in immune response of the cornea during the inflammatory process.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology11050793