RNF43 Mutations in IPMN Cases: A Potential Prognostic Factor

An intraductal papillary mucinous neoplasm (IPMN) is a common pancreatic precursor lesion, and it often harbors mutations in KRAS, GNAS, and RNF43. To clarify the molecular profiles of IPMNs, we conducted mutation analysis of KRAS, GNAS, and RNF43 in 61 IPMN formalin-fixed, paraffin-embedded (FFPE)...

Full description

Saved in:
Bibliographic Details
Published in:Gastroenterology research and practice 2020, Vol.2020 (2020), p.1-10
Main Authors: Wang, Peng Yan, Jiang, Ying, Wu, Yan, Chang, Xiao Yan, Chen, Jie
Format: Article
Language:English
Subjects:
Cancer
Classification
Deoxyribonucleic acid
DNA
Medical prognosis
Morphology
Mutation
Proteins
Survival analysis
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An intraductal papillary mucinous neoplasm (IPMN) is a common pancreatic precursor lesion, and it often harbors mutations in KRAS, GNAS, and RNF43. To clarify the molecular profiles of IPMNs, we conducted mutation analysis of KRAS, GNAS, and RNF43 in 61 IPMN formalin-fixed, paraffin-embedded (FFPE) specimens. The mutation rates of codons 12, 13, and 61 in KRAS and codon 201 in GNAS were detected by Sanger sequencing. Next-generation sequencing was performed on RNF43, and the results were further verified by Sanger sequencing. We identified KRAS and GNAS mutations in 35 (57%) and 40 (66%) IPMN cases, respectively. GNAS mutations were significantly correlated with the morphologic subtype (P
ISSN:1687-6121
1687-630X
DOI:10.1155/2020/1457452