AaTAS1 and AaMFS1 Genes for Biosynthesis or Efflux Transport of Tenuazonic Acid and Pathogenicity of Alternaria alternata

Taking tenuazonic acid (TeA) synthetase 1 (TAS1) in as a reference, the homolog 1 was first anchored in via de novo sequencing. Subsequently, , as a major facilitator superfamily (MFS) protein-encoding gene in the adjacent upstream region, was followed with interest. As hypothesized, AaTAS1 is requi...

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Published in:Molecular plant-microbe interactions 2022-05, Vol.35 (5), p.416-427
Main Authors: Sun, Fan, Cao, Xueqiang, Yu, Dianzhen, Hu, Dongqiang, Yan, Zheng, Fan, Yingying, Wang, Cheng, Wu, Aibo
Format: Article
Language:English
Subjects:
AaMFS1
AaTAS1
Alternaria alternata
Biosynthesis
Cytoplasm
Efflux
efflux transport
Fluorescence
Gene expression
Genes
Homology
Localization
Membranes
Mycotoxins
Pathogenicity
Pathogens
Phenotypes
Proteins
Tenuazonic acid
Transcriptomes
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Summary:Taking tenuazonic acid (TeA) synthetase 1 (TAS1) in as a reference, the homolog 1 was first anchored in via de novo sequencing. Subsequently, , as a major facilitator superfamily (MFS) protein-encoding gene in the adjacent upstream region, was followed with interest. As hypothesized, AaTAS1 is required for TeA biosynthesis, while AaMFS1 is an efflux pump for the transmembrane transport of TeA. Comparatively, the TeA yield of Δ and Δ dropped significantly compared with that of the wild-type strain. Specifically, the A domain of AaTAS1 catalyzed the start of TeA biosynthesis in vitro. Simultaneously, the pathogenicity of Δ was also significantly decreased. Transcriptome analysis confirmed the abovementioned consistency between the TeA-producing phenotypes and related gene expression. Moreover, the proteins AaTAS1 and AaMFS1 were found present in the cytoplasm, plasma membrane, and intracellular membrane system, respectively, by fluorescence localization. Namely, AaTAS1 was responsible for the biosynthesis of TeA, and AaMFS1 was responsible for the efflux transport of TeA. Certainly, indirectly regulated the expression of through the level of synthetic TeA. Overall, data on the novel 1 and genes mainly contribute to theoretical advances in mycotoxin biosynthesis and the pathogenicity of phytopathogens to agricultural foods.[Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
ISSN:0894-0282
1943-7706
DOI:10.1094/MPMI-12-21-0300-R