Single-cell profiling of the human decidual immune microenvironment in patients with recurrent pregnancy loss

Maintaining homeostasis of the decidual immune microenvironment at the maternal–fetal interface is essential for placentation and reproductive success. Although distinct decidual immune cell subpopulations have been identified under normal conditions, systematic understanding of the spectrum and het...

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Bibliographic Details
Published in:Cell discovery 2021-01, Vol.7 (1), p.1-1, Article 1
Main Authors: Guo, Chuang, Cai, Pengfei, Jin, Liying, Sha, Qing, Yu, Qiaoni, Zhang, Wen, Jiang, Chen, Liu, Qian, Zong, Dandan, Li, Kun, Fang, Jingwen, Lu, Fangting, Wang, Yanshi, Li, Daojing, Lin, Jun, Li, Lu, Zeng, Zhutian, Tong, Xianhong, Wei, Haiming, Qu, Kun
Format: Article
Language:English
Subjects:
631/1647/2217/2018
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Biomedical and Life Sciences
Breeding success
Cell Biology
Cell Culture
Cell Cycle Analysis
Cell Physiology
Decidua
Dendritic cells
Fetuses
Gene expression
Homeostasis
Leukocytes
Life Sciences
Lymphocytes
Microenvironments
Miscarriage
Natural killer cells
Pregnancy
Stem Cells
Stromal cells
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Summary:Maintaining homeostasis of the decidual immune microenvironment at the maternal–fetal interface is essential for placentation and reproductive success. Although distinct decidual immune cell subpopulations have been identified under normal conditions, systematic understanding of the spectrum and heterogeneity of leukocytes under recurrent miscarriage in human deciduas remains unclear. To address this, we profiled the respective transcriptomes of 18,646 primary human decidual immune cells isolated from patients with recurrent pregnancy loss (RPL) and healthy controls at single-cell resolution. We discovered dramatic differential distributions of immune cell subsets in RPL patients compared with the normal decidual immune microenvironment. Furthermore, we found a subset of decidual natural killer (NK) cells that support embryo growth were diminished in proportion due to abnormal NK cell development in RPL patients. We also elucidated the altered cellular interactions between the decidual immune cell subsets in the microenvironment and those of the immune cells with stromal cells and extravillous trophoblast under disease state. These results provided deeper insights into the RPL decidual immune microenvironment disorder that are potentially applicable to improve the diagnosis and therapeutics of this disease.
ISSN:2056-5968
2056-5968
DOI:10.1038/s41421-020-00236-z