Substrate Recognition and Specificity of Chitin Deacetylases and Related Family 4 Carbohydrate Esterases

Carbohydrate esterases family 4 (CE4 enzymes) includes chitin and peptidoglycan deacetylases, acetylxylan esterases, and poly- -acetylglucosamine deacetylases that act on structural polysaccharides, altering their physicochemical properties, and participating in diverse biological functions. Chitin...

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Bibliographic Details
Published in:International journal of molecular sciences 2018-01, Vol.19 (2), p.412
Main Authors: Aragunde, Hugo, Biarnés, Xevi, Planas, Antoni
Format: Article
Language:English
Subjects:
Bacteria
Biocatalysts
Biofilms
carbohydrate esterases
Carbohydrates
Cell walls
Chitin
chitin deacetylases
chitooligosaccharides
chitosan
Deacetylation
deacetylation pattern
Drug development
Enzymes
Esterases
Fungi
Microorganisms
Morphogenesis
N-Acetylglucosamine
peptidoglycan
Peptidoglycans
Physicochemical properties
Polysaccharides
Review
structure
Substrate specificity
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Summary:Carbohydrate esterases family 4 (CE4 enzymes) includes chitin and peptidoglycan deacetylases, acetylxylan esterases, and poly- -acetylglucosamine deacetylases that act on structural polysaccharides, altering their physicochemical properties, and participating in diverse biological functions. Chitin and peptidoglycan deacetylases are not only involved in cell wall morphogenesis and remodeling in fungi and bacteria, but they are also used by pathogenic microorganisms to evade host defense mechanisms. Likewise, biofilm formation in bacteria requires partial deacetylation of extracellular polysaccharides mediated by poly- -acetylglucosamine deacetylases. Such biological functions make these enzymes attractive targets for drug design against pathogenic fungi and bacteria. On the other side, acetylxylan esterases deacetylate plant cell wall complex xylans to make them accessible to hydrolases, making them attractive biocatalysts for biomass utilization. CE4 family members are metal-dependent hydrolases. They are highly specific for their particular substrates, and show diverse modes of action, exhibiting either processive, multiple attack, or patterned deacetylation mechanisms. However, the determinants of substrate specificity remain poorly understood. Here, we review the current knowledge on the structure, activity, and specificity of CE4 enzymes, focusing on chitin deacetylases and related enzymes active on -acetylglucosamine-containing oligo and polysaccharides.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms19020412