A role of lipin in human obesity and insulin resistance: relation to adipocyte glucose transport and GLUT4 expression

The mouse lipin gene, Lpin1, is important for adipose tissue development and is a candidate gene for insulin resistance. Here, we investigate the adipose tissue expression levels of the human LPIN1 gene in relation to various clinical variables as well as adipocyte function. LPIN1 gene expression wa...

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Bibliographic Details
Published in:Journal of lipid research 2007-01, Vol.48 (1), p.201-206
Main Authors: van Harmelen, Vanessa, Rydén, Mikael, Sjölin, Eva, Hoffstedt, Johan
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adipocytes - drug effects
Adipocytes - metabolism
Adipocytes - pathology
adipogenesis
adipose tissue
Adipose Tissue - metabolism
Cell Differentiation
Cohort Studies
fat cell
Gene Expression Regulation
Glucose - metabolism
Glucose Transporter Type 4 - genetics
Humans
Insulin - pharmacology
Insulin Resistance - genetics
Medicin och hälsovetenskap
metabolic syndrome
mRNA
Nuclear Proteins - genetics
Obesity - blood
Obesity - genetics
Phosphatidate Phosphatase
RNA, Messenger - genetics
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Summary:The mouse lipin gene, Lpin1, is important for adipose tissue development and is a candidate gene for insulin resistance. Here, we investigate the adipose tissue expression levels of the human LPIN1 gene in relation to various clinical variables as well as adipocyte function. LPIN1 gene expression was induced at an early step in human preadipocyte differentiation in parallel with peroxisome proliferator-activated receptor γ. Lipin mRNA levels were higher in fat cells than in adipose tissue segments but showed no difference between subcutaneous and omental depots. Moreover, LPIN1 expression levels were reduced in obesity, improved following weight reduction in obese subjects, and were downregulated in women with the metabolic syndrome. With respect to adipocyte function, adipose LPIN1 gene expression was strongly associated with both basal and insulin-mediated subcutaneous adipocyte glucose transport as well as mRNA levels of glucose transporter 4 (GLUT4). We show that body fat accumulation is a major regulator of human adipose LPIN1 expression and suggest a role of LPIN1 in human preadipocyte as well as mature adipocyte function.
ISSN:0022-2275
1539-7262
DOI:10.1194/jlr.M600272-JLR200