SnRK1-triggered switch of bZIP63 dimerization mediates the low-energy response in plants

Metabolic adjustment to changing environmental conditions, particularly balancing of growth and defense responses, is crucial for all organisms to survive. The evolutionary conserved AMPK/Snf1/SnRK1 kinases are well-known metabolic master regulators in the low-energy response in animals, yeast and p...

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Published in:eLife 2015-08, Vol.4
Main Authors: Mair, Andrea, Pedrotti, Lorenzo, Wurzinger, Bernhard, Anrather, Dorothea, Simeunovic, Andrea, Weiste, Christoph, Valerio, Concetta, Dietrich, Katrin, Kirchler, Tobias, Nägele, Thomas, Vicente Carbajosa, Jesús, Hanson, Johannes, Baena-González, Elena, Chaban, Christina, Weckwerth, Wolfram, Dröge-Laser, Wolfgang, Teige, Markus
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - metabolism
Basic-Leucine Zipper Transcription Factors - deficiency
Basic-Leucine Zipper Transcription Factors - metabolism
bZIP transcription factor
Cell Biology
Gene Expression Regulation, Plant
Gene Knockout Techniques
Genetic Complementation Test
metabolic reprogramming
Phosphorylation
Plant Biology
Protein Multimerization
Protein Processing, Post-Translational
Protein-Serine-Threonine Kinases - metabolism
SnRK1 kinase
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Summary:Metabolic adjustment to changing environmental conditions, particularly balancing of growth and defense responses, is crucial for all organisms to survive. The evolutionary conserved AMPK/Snf1/SnRK1 kinases are well-known metabolic master regulators in the low-energy response in animals, yeast and plants. They act at two different levels: by modulating the activity of key metabolic enzymes, and by massive transcriptional reprogramming. While the first part is well established, the latter function is only partially understood in animals and not at all in plants. Here we identified the Arabidopsis transcription factor bZIP63 as key regulator of the starvation response and direct target of the SnRK1 kinase. Phosphorylation of bZIP63 by SnRK1 changed its dimerization preference, thereby affecting target gene expression and ultimately primary metabolism. A bzip63 knock-out mutant exhibited starvation-related phenotypes, which could be functionally complemented by wild type bZIP63, but not by a version harboring point mutations in the identified SnRK1 target sites.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.05828