Advancing osteoarthritis therapy with GMOCS hydrogel-loaded BMSCs-exos

This study investigated the mechanism of the extracellular matrix-mimicking hydrogel-mediated TGFB1/Nrf2 signaling pathway in osteoarthritis using bone marrow mesenchymal stem cell-derived exosomes (BMSCs-Exos). A GMOCS-Exos hydrogel was synthesized and evaluated for its impact on chondrocyte viabil...

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Bibliographic Details
Published in:Journal of nanobiotechnology 2024-08, Vol.22 (1), p.493-24, Article 493
Main Authors: Zhou, Renyi, Guo, Jiarong, Jin, Zhe
Format: Article
Language:English
Subjects:
Animals
Care and treatment
Cartilage regeneration
Cell organelles
Cell Survival - drug effects
Cells, Cultured
Cellular therapy
Chondrocytes - metabolism
Colloids in medicine
Disease Models, Animal
Ethylenediaminetetraacetic acid
Exosomes
Exosomes - metabolism
Extracellular Traps - metabolism
Health aspects
Humans
Hydrogel
Hydrogels - chemistry
Male
Materials
Mesenchymal Stem Cells - metabolism
Methods
NF-E2-Related Factor 2 - metabolism
Nrf2 signaling
Osteoarthritis
Osteoarthritis - therapy
Rats
Rats, Sprague-Dawley
Scientific equipment and supplies industry
Signal Transduction
Stem cells
TGFB1
Transforming Growth Factor beta1 - metabolism
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Summary:This study investigated the mechanism of the extracellular matrix-mimicking hydrogel-mediated TGFB1/Nrf2 signaling pathway in osteoarthritis using bone marrow mesenchymal stem cell-derived exosomes (BMSCs-Exos). A GMOCS-Exos hydrogel was synthesized and evaluated for its impact on chondrocyte viability and neutrophil extracellular traps (NETs) formation. In an OA rat model, GMOCS-Exos promoted cartilage regeneration and inhibited NETs formation. Transcriptome sequencing identified TGFB1 as a key gene, with GMOCS-Exos activating Nrf2 signaling through TGFB1. Depletion of TGFB1 hindered the cartilage-protective effect of GMOCS-Exos. This study sheds light on a promising therapeutic strategy for osteoarthritis through GMOCS-Exos-mediated TGFB1/Nrf2 pathway modulation.
ISSN:1477-3155
1477-3155
DOI:10.1186/s12951-024-02713-z