Effect of introduction of chondroitin sulfate into polymer-peptide conjugate responding to intracellular signals

We recently developed a novel tumor-targeted gene delivery system responding to hyperactivated intracellular signals. Polymeric carrier for gene delivery consists of hydrophilic neutral polymer as main chains and cationic peptide substrate for target enzyme as side chains, and was named polymer-pept...

Full description

Saved in:
Bibliographic Details
Published in:Nanoscale research letters 2011-09, Vol.6 (1), p.532-532, Article 532
Main Authors: Tomiyama, Tetsuro, Toita, Riki, Kang, Jeong-Hun, Koga, Haruka, Shiosaki, Shujiro, Mori, Takeshi, Niidome, Takuro, Katayama, Yoshiki
Format: Article
Language:English
Subjects:
Chemistry and Materials Science
Materials Science
Molecular Medicine
Nano Express
Nanochemistry
Nanoscale Science and Technology
Nanotechnology
Nanotechnology and Microengineering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-b558t-86c5d8c102feac6dac8a7c34252d8e833691abe1500abd67dbe2c371cb1114ae3
cites cdi_FETCH-LOGICAL-b558t-86c5d8c102feac6dac8a7c34252d8e833691abe1500abd67dbe2c371cb1114ae3
container_end_page 532
container_issue 1
container_start_page 532
container_title Nanoscale research letters
container_volume 6
creator Tomiyama, Tetsuro
Toita, Riki
Kang, Jeong-Hun
Koga, Haruka
Shiosaki, Shujiro
Mori, Takeshi
Niidome, Takuro
Katayama, Yoshiki
description We recently developed a novel tumor-targeted gene delivery system responding to hyperactivated intracellular signals. Polymeric carrier for gene delivery consists of hydrophilic neutral polymer as main chains and cationic peptide substrate for target enzyme as side chains, and was named polymer-peptide conjugate (PPC). Introduction of chondroitin sulfate (CS), which induces receptor-medicated endocytosis, into polymers mainly with a high cationic charge density such as polyethylenimine can increase tumor-targeted gene delivery. In the present study, we examined whether introduction of CS into PPC containing five cationic amino acids can increase gene expression in tumor cells. Size and zeta potential of plasmid DNA (pDNA)/PPC/CS complex were
doi_str_mv 10.1186/1556-276X-6-532
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_cd672c960ac14a8e94dde2ab56454f9a</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_cd672c960ac14a8e94dde2ab56454f9a</doaj_id><sourcerecordid>900642381</sourcerecordid><originalsourceid>FETCH-LOGICAL-b558t-86c5d8c102feac6dac8a7c34252d8e833691abe1500abd67dbe2c371cb1114ae3</originalsourceid><addsrcrecordid>eNp1kkFv1DAQhSMEoqVw5oZy4xRqO7HjXJCgKrRSJS4gcbOc8ST1KmsHO6nUf49NyqortaexZ9589vi5KN5T8olSKc4p56JirfhdiYrX7EVxesi8fLQ-Kd7EuCOkaUkrXhcnjHaCyqY-LebLYUBYSj-U1i3BmxUW613ew613Jni7WFfGdRr0glnjy9lP93sM1YzzYg2W4N1uHXM5YJxTk3VjmXQZqAGnaZ10KKMdnZ7i2-LVkAK-e4hnxa9vlz8vrqqbH9-vL77cVD3ncqmkAG4kUMIG1CCMBqlbqBvGmZEo61p0VPdIOSG6N6I1PTKoWwo9pbTRWJ8V1xvXeL1Tc7B7He6V11b9S_gwKh0WCxMqSP0MOkE0pFaJXWMMMt1z0fBm6HRifd5Y89rv0QDmwaYj6HHF2Vs1-jtVM8pISxLg6wborX8GcFwBv1fZPZXdU0IlbxPk48Mtgv-zYlzU3sb8vNqhX6PqCBENqyVNyvNNCcHHGHA4nESJyv_mCfaHxxMe9P8_ShKQTRBTyY0Y1M6vIRv6LPMvLaHS9g</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>900642381</pqid></control><display><type>article</type><title>Effect of introduction of chondroitin sulfate into polymer-peptide conjugate responding to intracellular signals</title><source>Publicly Available Content Database</source><source>IngentaConnect Journals</source><source>PubMed Central</source><creator>Tomiyama, Tetsuro ; Toita, Riki ; Kang, Jeong-Hun ; Koga, Haruka ; Shiosaki, Shujiro ; Mori, Takeshi ; Niidome, Takuro ; Katayama, Yoshiki</creator><creatorcontrib>Tomiyama, Tetsuro ; Toita, Riki ; Kang, Jeong-Hun ; Koga, Haruka ; Shiosaki, Shujiro ; Mori, Takeshi ; Niidome, Takuro ; Katayama, Yoshiki</creatorcontrib><description>We recently developed a novel tumor-targeted gene delivery system responding to hyperactivated intracellular signals. Polymeric carrier for gene delivery consists of hydrophilic neutral polymer as main chains and cationic peptide substrate for target enzyme as side chains, and was named polymer-peptide conjugate (PPC). Introduction of chondroitin sulfate (CS), which induces receptor-medicated endocytosis, into polymers mainly with a high cationic charge density such as polyethylenimine can increase tumor-targeted gene delivery. In the present study, we examined whether introduction of CS into PPC containing five cationic amino acids can increase gene expression in tumor cells. Size and zeta potential of plasmid DNA (pDNA)/PPC/CS complex were &lt;200 nm and between -10 and -15 mV, respectively. In tumor cell experiments, pDNA/PPC/CS complex showed lower stability and gene regulation, compared with that of pDNA/PPC. Moreover, no difference in gene expression was identified between positive and negative polymer. These results were caused by fast disintegration of pDNA/PPC/CS complexes in the presence of serum. Thus, we suggest that introduction of negatively charged CS into polymers with a low charge density may lead to low stability and gene regulation of complexes.</description><identifier>ISSN: 1556-276X</identifier><identifier>ISSN: 1931-7573</identifier><identifier>EISSN: 1556-276X</identifier><identifier>DOI: 10.1186/1556-276X-6-532</identifier><identifier>PMID: 21961843</identifier><language>eng</language><publisher>New York: Springer New York</publisher><subject>Chemistry and Materials Science ; Materials Science ; Molecular Medicine ; Nano Express ; Nanochemistry ; Nanoscale Science and Technology ; Nanotechnology ; Nanotechnology and Microengineering</subject><ispartof>Nanoscale research letters, 2011-09, Vol.6 (1), p.532-532, Article 532</ispartof><rights>Tomiyama et al; licensee Springer. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright ©2011 Tomiyama et al; licensee Springer. 2011 Tomiyama et al; licensee Springer.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b558t-86c5d8c102feac6dac8a7c34252d8e833691abe1500abd67dbe2c371cb1114ae3</citedby><cites>FETCH-LOGICAL-b558t-86c5d8c102feac6dac8a7c34252d8e833691abe1500abd67dbe2c371cb1114ae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212070/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212070/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,37013,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21961843$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tomiyama, Tetsuro</creatorcontrib><creatorcontrib>Toita, Riki</creatorcontrib><creatorcontrib>Kang, Jeong-Hun</creatorcontrib><creatorcontrib>Koga, Haruka</creatorcontrib><creatorcontrib>Shiosaki, Shujiro</creatorcontrib><creatorcontrib>Mori, Takeshi</creatorcontrib><creatorcontrib>Niidome, Takuro</creatorcontrib><creatorcontrib>Katayama, Yoshiki</creatorcontrib><title>Effect of introduction of chondroitin sulfate into polymer-peptide conjugate responding to intracellular signals</title><title>Nanoscale research letters</title><addtitle>Nanoscale Res Lett</addtitle><addtitle>Nanoscale Res Lett</addtitle><description>We recently developed a novel tumor-targeted gene delivery system responding to hyperactivated intracellular signals. Polymeric carrier for gene delivery consists of hydrophilic neutral polymer as main chains and cationic peptide substrate for target enzyme as side chains, and was named polymer-peptide conjugate (PPC). Introduction of chondroitin sulfate (CS), which induces receptor-medicated endocytosis, into polymers mainly with a high cationic charge density such as polyethylenimine can increase tumor-targeted gene delivery. In the present study, we examined whether introduction of CS into PPC containing five cationic amino acids can increase gene expression in tumor cells. Size and zeta potential of plasmid DNA (pDNA)/PPC/CS complex were &lt;200 nm and between -10 and -15 mV, respectively. In tumor cell experiments, pDNA/PPC/CS complex showed lower stability and gene regulation, compared with that of pDNA/PPC. Moreover, no difference in gene expression was identified between positive and negative polymer. These results were caused by fast disintegration of pDNA/PPC/CS complexes in the presence of serum. Thus, we suggest that introduction of negatively charged CS into polymers with a low charge density may lead to low stability and gene regulation of complexes.</description><subject>Chemistry and Materials Science</subject><subject>Materials Science</subject><subject>Molecular Medicine</subject><subject>Nano Express</subject><subject>Nanochemistry</subject><subject>Nanoscale Science and Technology</subject><subject>Nanotechnology</subject><subject>Nanotechnology and Microengineering</subject><issn>1556-276X</issn><issn>1931-7573</issn><issn>1556-276X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp1kkFv1DAQhSMEoqVw5oZy4xRqO7HjXJCgKrRSJS4gcbOc8ST1KmsHO6nUf49NyqortaexZ9589vi5KN5T8olSKc4p56JirfhdiYrX7EVxesi8fLQ-Kd7EuCOkaUkrXhcnjHaCyqY-LebLYUBYSj-U1i3BmxUW613ew613Jni7WFfGdRr0glnjy9lP93sM1YzzYg2W4N1uHXM5YJxTk3VjmXQZqAGnaZ10KKMdnZ7i2-LVkAK-e4hnxa9vlz8vrqqbH9-vL77cVD3ncqmkAG4kUMIG1CCMBqlbqBvGmZEo61p0VPdIOSG6N6I1PTKoWwo9pbTRWJ8V1xvXeL1Tc7B7He6V11b9S_gwKh0WCxMqSP0MOkE0pFaJXWMMMt1z0fBm6HRifd5Y89rv0QDmwaYj6HHF2Vs1-jtVM8pISxLg6wborX8GcFwBv1fZPZXdU0IlbxPk48Mtgv-zYlzU3sb8vNqhX6PqCBENqyVNyvNNCcHHGHA4nESJyv_mCfaHxxMe9P8_ShKQTRBTyY0Y1M6vIRv6LPMvLaHS9g</recordid><startdate>20110930</startdate><enddate>20110930</enddate><creator>Tomiyama, Tetsuro</creator><creator>Toita, Riki</creator><creator>Kang, Jeong-Hun</creator><creator>Koga, Haruka</creator><creator>Shiosaki, Shujiro</creator><creator>Mori, Takeshi</creator><creator>Niidome, Takuro</creator><creator>Katayama, Yoshiki</creator><general>Springer New York</general><general>BioMed Central Ltd</general><general>Springer</general><general>SpringerOpen</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20110930</creationdate><title>Effect of introduction of chondroitin sulfate into polymer-peptide conjugate responding to intracellular signals</title><author>Tomiyama, Tetsuro ; Toita, Riki ; Kang, Jeong-Hun ; Koga, Haruka ; Shiosaki, Shujiro ; Mori, Takeshi ; Niidome, Takuro ; Katayama, Yoshiki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b558t-86c5d8c102feac6dac8a7c34252d8e833691abe1500abd67dbe2c371cb1114ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Chemistry and Materials Science</topic><topic>Materials Science</topic><topic>Molecular Medicine</topic><topic>Nano Express</topic><topic>Nanochemistry</topic><topic>Nanoscale Science and Technology</topic><topic>Nanotechnology</topic><topic>Nanotechnology and Microengineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tomiyama, Tetsuro</creatorcontrib><creatorcontrib>Toita, Riki</creatorcontrib><creatorcontrib>Kang, Jeong-Hun</creatorcontrib><creatorcontrib>Koga, Haruka</creatorcontrib><creatorcontrib>Shiosaki, Shujiro</creatorcontrib><creatorcontrib>Mori, Takeshi</creatorcontrib><creatorcontrib>Niidome, Takuro</creatorcontrib><creatorcontrib>Katayama, Yoshiki</creatorcontrib><collection>SpringerOpen(OpenAccess)</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Nanoscale research letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tomiyama, Tetsuro</au><au>Toita, Riki</au><au>Kang, Jeong-Hun</au><au>Koga, Haruka</au><au>Shiosaki, Shujiro</au><au>Mori, Takeshi</au><au>Niidome, Takuro</au><au>Katayama, Yoshiki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of introduction of chondroitin sulfate into polymer-peptide conjugate responding to intracellular signals</atitle><jtitle>Nanoscale research letters</jtitle><stitle>Nanoscale Res Lett</stitle><addtitle>Nanoscale Res Lett</addtitle><date>2011-09-30</date><risdate>2011</risdate><volume>6</volume><issue>1</issue><spage>532</spage><epage>532</epage><pages>532-532</pages><artnum>532</artnum><issn>1556-276X</issn><issn>1931-7573</issn><eissn>1556-276X</eissn><abstract>We recently developed a novel tumor-targeted gene delivery system responding to hyperactivated intracellular signals. Polymeric carrier for gene delivery consists of hydrophilic neutral polymer as main chains and cationic peptide substrate for target enzyme as side chains, and was named polymer-peptide conjugate (PPC). Introduction of chondroitin sulfate (CS), which induces receptor-medicated endocytosis, into polymers mainly with a high cationic charge density such as polyethylenimine can increase tumor-targeted gene delivery. In the present study, we examined whether introduction of CS into PPC containing five cationic amino acids can increase gene expression in tumor cells. Size and zeta potential of plasmid DNA (pDNA)/PPC/CS complex were &lt;200 nm and between -10 and -15 mV, respectively. In tumor cell experiments, pDNA/PPC/CS complex showed lower stability and gene regulation, compared with that of pDNA/PPC. Moreover, no difference in gene expression was identified between positive and negative polymer. These results were caused by fast disintegration of pDNA/PPC/CS complexes in the presence of serum. Thus, we suggest that introduction of negatively charged CS into polymers with a low charge density may lead to low stability and gene regulation of complexes.</abstract><cop>New York</cop><pub>Springer New York</pub><pmid>21961843</pmid><doi>10.1186/1556-276X-6-532</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1556-276X
ispartof Nanoscale research letters, 2011-09, Vol.6 (1), p.532-532, Article 532
issn 1556-276X
1931-7573
1556-276X
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_cd672c960ac14a8e94dde2ab56454f9a
source Publicly Available Content Database; IngentaConnect Journals; PubMed Central
subjects Chemistry and Materials Science
Materials Science
Molecular Medicine
Nano Express
Nanochemistry
Nanoscale Science and Technology
Nanotechnology
Nanotechnology and Microengineering
title Effect of introduction of chondroitin sulfate into polymer-peptide conjugate responding to intracellular signals
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T06%3A08%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20introduction%20of%20chondroitin%20sulfate%20into%20polymer-peptide%20conjugate%20responding%20to%20intracellular%20signals&rft.jtitle=Nanoscale%20research%20letters&rft.au=Tomiyama,%20Tetsuro&rft.date=2011-09-30&rft.volume=6&rft.issue=1&rft.spage=532&rft.epage=532&rft.pages=532-532&rft.artnum=532&rft.issn=1556-276X&rft.eissn=1556-276X&rft_id=info:doi/10.1186/1556-276X-6-532&rft_dat=%3Cproquest_doaj_%3E900642381%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-b558t-86c5d8c102feac6dac8a7c34252d8e833691abe1500abd67dbe2c371cb1114ae3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=900642381&rft_id=info:pmid/21961843&rfr_iscdi=true