Ethnobotanical Identification and Anti-microbial Evaluation of Some Anti- Diarrhoreal Plants Used by the Samburu Community, Kenya

• Published in: Malaysian Journal of Microbiology 2012-06, Vol.8 (2), p.68-74
• Main Authors: Omori, E. O., Calistus, O., Mbugua, P. K., Okemo, P. O.
• Format: Article
• Language: English
• Subjects: antimicrobial activity; diarrhoea; ethnobotany; medicinal plants; phytochemicals
• ISSN: 1823-8262; 2231-7538

Aims:The anti-bacterial effect of some selected Samburu medicinal plants were evaluated on bacterial strains like Staphylococcus aureus-ATCC 20591, Bacillus subtillis-local isolate, Salmonella typhi-ATCC 2202, Escherichia coli-STD-25922 and Pseudomonas aeruginosa-ATCC 25852.Methodology and Results: Methanol was used as an extraction solvent for the medicinal plants after they had beendried and ground using the laboratory grinding miller. The in vitro anti-bacterial activity was performed by agar disc diffusion method. The significant anti-microbial activity of active extracts was compared with the standard antibiotics,cefrodoxima and amoxicillin. These results were significant at p < 0.01. The MICs were also determined by the microtitre-plate method. The MICs of the most active plants ranged from 18.75 mg/mL to 37.5 mg/mL. The MBCs rangedbetween 18.75 mg/mL to 37.5 mg/mL. The most active plant that had substantial activity in at least all the test cultures was found to be Lannea triphylla (A.Rich) Engl. A number of phytochemicals were also found to be present with tannins being the most abundant followed by flavonoids and saponins.Conclusion, significance and impact of study: The findings show that most of the medicinal plants used by theSamburu community have some significant activity on the bacterial isolates known to cause diarrhoea.