Long chain arabinoxylans shift the mucosa-associated microbiota in the proximal colon of the simulator of the human intestinal microbial ecosystem (M-SHIME)

• Published in: Journal of functional foods 2017-05, Vol.32, p.226-237
• Main Authors: Truchado, Pilar, Hernandez-Sanabria, Emma, Salden, Bouke N., Van den Abbeele, Pieter, Vilchez-Vargas, Ramiro, Jauregui, Ruy, Pieper, Dietmar H., Possemiers, Sam, Van de Wiele, Tom
• Format: Article
• Language: English
• Subjects: Bifidobacterium; In vitro; Microbiome; Mucus; Prebiotics; Propionate
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2017.02.004

•LC-AX modulated lumen and mucus microbiota of proximal colon (M-SHIME).•LC-AX modulated gut microbiota, consistent with inter-individual variations.•LC-AX increased Bifidobacterium in both lumen and mucus compartments from M-SHIME.•LC-AX had a potential prebiotic effect in the mucus layer.•High dose of LC- AX increased propionate in the in vitro proximal colon. Prebiotic compounds aim at improving human health by targeting beneficial gut microbes. We used a dynamic in vitro model of the human digestive tract (M-SHIME®) to study the modulatory effects of long-chain arabinoxylans (LC-AX) towards luminal and mucosal microbiota. Two doses (3 and 6g/L) of LC-AX were supplied to the proximal colon compartments of the M-SHIME, three times per day for 3days. Next generation sequencing and multivariate statistical analysis revealed the distribution of key microbial genera across M-SHIME compartments. Supplementation of 6g/L of LC-AX significantly increased the relative abundance of Bifidobacterium in both lumen (from 0.34% to 0.41%, 1.2-fold) and mucus (from 0.14% to 0.29%, 2.1-fold) compared with the control (P