Transcriptomic landscape of the interaction between the entomopathogenic fungus Beauveria bassiana and its tolerant host Tribolium castaneum revealed by dual RNA-seq

Entomopathogenic fungi such as Beauveria bassiana are the only insect pathogens able to start the infection process by penetrating through the host cuticle. However, some insects try to avoid fungal infection by embedding their cuticle with antifungal compounds. This is the case of the red flour bee...

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Bibliographic Details
Published in:Scientific reports 2023-10, Vol.13 (1), p.16506-16506, Article 16506
Main Authors: Mannino, María Constanza, Davyt-Colo, Belén, Huarte-Bonnet, Carla, Diambra, Luis, Pedrini, Nicolás
Format: Article
Language:English
Subjects:
631/114/2114
631/1647/48
Beauveria bassiana
Catabolite repression
Cellular stress response
Chitin
Conidia
Embedding
Entomopathogenic fungi
Epicuticle
Fungal infections
Fungi
Gene mapping
Genomes
Humanities and Social Sciences
Insects
multidisciplinary
Science
Science (multidisciplinary)
Transcriptomics
Tribolium castaneum
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Summary:Entomopathogenic fungi such as Beauveria bassiana are the only insect pathogens able to start the infection process by penetrating through the host cuticle. However, some insects try to avoid fungal infection by embedding their cuticle with antifungal compounds. This is the case of the red flour beetle Tribolium castaneum , which generates economical loss of great significance in stored product environments worldwide. In this study, T. castaneum adults were fed during different time periods (from 3 to 72 h) on B. bassiana conidia-covered corn kernels. The progression of fungal infection was monitored using the dual RNA-seq technique to reconstruct the temporal transcriptomic profile and to perform gene enrichment analyses in both interacting organisms. After mapping the total reads with the B. bassiana genome, 904 genes were identified during this process. The more expressed fungal genes were related to carbon catabolite repression, cation binding, peptidase inhibition, redox processes, and stress response. Several immune-related genes from Toll, IMD, and JNK pathways, as well as genes related to chitin modification, were found to be differentially expressed in fungus-exposed T. castaneum . This study represents the first dual transcriptomic approach to help understand the interaction between the entomopathogenic fungus B. bassiana and its tolerant host T. castaneum .
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-43889-y