High-Yield Characterization of Single Molecule Interactions with DeepTipTM Atomic Force Microscopy Probes

Single molecule interactions between biotin and streptavidin were characterized with functionalized DeepTipTM probes and used as a model system to develop a comprehensive methodology for the high-yield identification and analysis of single molecular events. The procedure comprises the covalent bindi...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2022-12, Vol.28 (1), p.226
Main Authors: Corregidor, Daniel, Tabraue, Raquel, Colchero, Luis, Daza, Rafael, Elices, Manuel, Guinea, Gustavo V., Pérez-Rigueiro, José
Format: Article
Language:English
Subjects:
affinity atomic force microscopy
AFM
Atomic force microscopy
Biotin
Chemical speciation
Experiments
functionalization
Low level
Microscopy
Probes
Reproducibility
Sensors
Silicon nitride
Streptavidin
Topography
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Summary:Single molecule interactions between biotin and streptavidin were characterized with functionalized DeepTipTM probes and used as a model system to develop a comprehensive methodology for the high-yield identification and analysis of single molecular events. The procedure comprises the covalent binding of the target molecule to a surface and of the sensing molecule to the DeepTipTM probe, so that the interaction between both chemical species can be characterized by obtaining force–displacement curves in an atomic force microscope. It is shown that molecular resolution is consistently attained with a percentage of successful events higher than 90% of the total number of recorded curves, and a very low level of unspecific interactions. The combination of both features is a clear indication of the robustness and versatility of the proposed methodology.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28010226