Alteration of tight junction gene expression by calcium- and vitamin D-deficient diet in the duodenum of calbindin-null mice

Calcium absorption is regulated by both active (transcellular) and passive (paracellular) pathways. Although each pathway has been studied, correlations between the two pathways have not been well elucidated. In previous investigations, the critical transcellular proteins, calbindin-D9k (CaBP-9k) an...

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Published in:International journal of molecular sciences 2013-11, Vol.14 (11), p.22997-23010
Main Authors: Hwang, Inho, Yang, Hyun, Kang, Hong-Seok, Ahn, Changhwan, Hong, Eui-Ju, An, Beum-Soo, Jeung, Eui-Bae
Format: Article
Language:English
Subjects:
Animals
calbindin
Calbindins - genetics
Calbindins - metabolism
calcium absorption
Calcium, Dietary - administration & dosage
Calcium, Dietary - metabolism
Diet
Duodenum - metabolism
Gene expression
Gene Expression Regulation
Mice
Mice, Knockout
paracellular pathway
Permeability
Proteins
Small intestine
tight junction
Tight Junctions - genetics
transcellular pathway
Veterinary medicine
Vitamin D
Vitamin D - administration & dosage
Vitamin D - metabolism
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Summary:Calcium absorption is regulated by both active (transcellular) and passive (paracellular) pathways. Although each pathway has been studied, correlations between the two pathways have not been well elucidated. In previous investigations, the critical transcellular proteins, calbindin-D9k (CaBP-9k) and -D28k (CaBP-28k), were shown to affect other transcellular pathways by buffering intracellular calcium concentrations. The rate of paracellular calcium transport in the duodenum is generally determined by the expression of tight junction genes. In the present study, the effect of dietary calcium and/or vitamin D supplementation on the expression of tight junction genes (occludin, ZO-1 and claudin 2, 10b, 12 and 15) in the duodenum of CaBP-9k- and/or -28k-deficient mice was examined. With a normal diet, the expression of most tight junction genes in the duodenum was significantly increased in CaBP-9k knockout (KO) mice compared to wild-type (WT) animals. With a calcium- and vitamin D-deficient diet, tight junction gene expression was significantly decreased in the duodenum of the CaBP-9k KO mice. These findings suggest that expression of paracellular tight junction genes is regulated by transcellular CaBP proteins, suggesting that active and passive calcium transport pathways may function cooperatively.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms141122997