The sodium/ascorbic acid co-transporter SVCT2 distributes in a striated membrane-enriched domain at the M-band level in slow-twitch skeletal muscle fibers

• Published in: Biological research 2024-11, Vol.57 (1), p.79-15, Article 79
• Main Authors: Sandoval, Daniel, Mella, Jessica, Ojeda, Jorge, Bermedo-García, Francisca, Low, Marcela, Marcellini, Sylvain, Castro, Maite A, Casas, Mariana, Jaimovich, Enrique, Henríquez, Juan Pablo
• Format: Article
• Language: English
• Subjects: Acids; Analysis; Animals; Antibiotics; Antioxidants; Aprotinin; Ascorbic acid; Ascorbic Acid - metabolism; Bioethics; Cell differentiation; Cells, Cultured; Chickens; Detergents; Detergents, Synthetic; Electrical stimuli; Embryos; Endoplasmic reticulum; Ethylenediaminetetraacetic acid; Fractionation; Homeostasis; Humans; Immunohistochemistry; Intracellular; Localization; M-band; Mice; Monoclonal antibodies; Muscle Fibers, Skeletal - metabolism; Muscle Fibers, Slow-Twitch - metabolism; Muscles; Musculoskeletal system; Myoblasts; Myotubes; Plasma; Proteins; Sarcoplasmic reticulum; Skeletal muscle; Sodium-Coupled Vitamin C Transporters - metabolism; SVCT2; Vitamin C; Vitamins
• ISSN: 0717-6287; 0716-9760; 0717-6287
• DOI: 10.1186/s40659-024-00554-6

Vitamin C plays key roles in cellular homeostasis, functioning as a potent antioxidant and a positive regulator of cell differentiation. In skeletal muscle, the vitamin C/sodium co-transporter SVCT2 is preferentially expressed in oxidative slow fibers. SVCT2 is up-regulated during the early fusion of primary myoblasts and decreases during initial myotube growth, indicating the relevance of vitamin C uptake via SVCT2 for early skeletal muscle differentiation and fiber-type definition. However, our understanding of SVCT2 expression and function in adult skeletal muscles is still limited. In this study, we demonstrate that SVCT2 exhibits an intracellular distribution in chicken slow skeletal muscles, following a highly organized striated pattern. A similar distribution was observed in human muscle samples, chicken cultured myotubes, and isolated mouse myofibers. Immunohistochemical analyses, combined with biochemical cell fractionation experiments, reveal a strong co-localization of SVCT2 with intracellular detergent-soluble membrane fractions at the central sarcomeric M-band, where it co-solubilizes with sarcoplasmic reticulum proteins. Remarkably, electrical stimulation of cultured myofibers induces the redistribution of SVCT2 into a vesicular pattern. Our results provide novel insights into the dynamic roles of SVCT2 in different intracellular compartments in response to functional demands.