3D Media Stabilizes Membrane and Prolongs Lifespan of Defolliculated Xenopus laevis Oocytes

oocytes are commonly used in many fundamental biological studies. One of the major limitations of oocytes is their short storage lifespan with most defolliculated oocytes physically deteriorating in 10 days or less. Herein, we identified a 3D Cultrex-based storage media that incorporates extracellul...

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Published in:Membranes (Basel) 2022-07, Vol.12 (8), p.754
Main Authors: Aggarwal, Nikhil D, Zeng, Steven L, Lashgari, Ryan J, Sudlow, Leland C, Berezin, Mikhail Y
Format: Article
Language:English
Subjects:
Communication
Cultrex
Experiments
Gametocytes
Gene expression
Hydrogels
Integrity
Life span
lifespan
Membranes
mRNA
Oocytes
Ovaries
Photonics
Plasmids
vitelline membrane
Xenopus laevis
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cited_by cdi_FETCH-LOGICAL-c493t-afc41ae1df0addd19b5f2a059f938357fc7fd7afbdd7f4e880fa7bda272cd68b3
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container_issue 8
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container_title Membranes (Basel)
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creator Aggarwal, Nikhil D
Zeng, Steven L
Lashgari, Ryan J
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Berezin, Mikhail Y
description oocytes are commonly used in many fundamental biological studies. One of the major limitations of oocytes is their short storage lifespan with most defolliculated oocytes physically deteriorating in 10 days or less. Herein, we identified a 3D Cultrex-based storage media that incorporates extracellular membrane-based hydrogels to maintain oocyte integrity. Under these treatments, the lifespan of the oocytes increased to more than 20 days compared to standard conditions. The treatment preserved the oocytes membrane integrity and did not interfere with mRNA- or cDNA-derived protein expression.
doi_str_mv 10.3390/membranes12080754
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subjects Communication
Cultrex
Experiments
Gametocytes
Gene expression
Hydrogels
Integrity
Life span
lifespan
Membranes
mRNA
Oocytes
Ovaries
Photonics
Plasmids
vitelline membrane
Xenopus laevis
title 3D Media Stabilizes Membrane and Prolongs Lifespan of Defolliculated Xenopus laevis Oocytes
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