LAMP real-time turbidity detection for fowl adenovirus

Fowl adenovirus (FAdV) is an infectious agent that can cause jaundice, severe anaemia, dyspnoea and reproductive disorders in fowls. Since 2015, FAdV disease has been rapidly spreading among broiler chickens in China, where it has caused significant economic losses. In this study, a loop-mediated is...

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Bibliographic Details
Published in:BMC veterinary research 2019-07, Vol.15 (1), p.256-256, Article 256
Main Authors: Yuan, Xiao-Yuan, Wang, You-Ling, Meng, Kai, Zhang, Yu-Xia, Xu, Huai-Ying, Ai, Wu
Format: Article
Language:English
Subjects:
Adenovirus
Adenovirus infections
Adenoviruses
Anemia
Broilers (Chickens)
Control
Disease transmission
DNA
Economic aspects
EDTA
Health aspects
Jaundice
LAMP
Methodology
Polymerase chain reaction
Prevention
Real-time turbidity
Risk factors
Visual detection
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Summary:Fowl adenovirus (FAdV) is an infectious agent that can cause jaundice, severe anaemia, dyspnoea and reproductive disorders in fowls. Since 2015, FAdV disease has been rapidly spreading among broiler chickens in China, where it has caused significant economic losses. In this study, a loop-mediated isothermal amplification (LAMP) real-time turbidity method with strong specificity to FAdV was established. The established assay was specific to FAdV-4, and the lower limit of detection was 75 copies/μL of extracted DNA. The positive detection rate for the suspected tissue samples was 33.3% (14/42), which was consistent with that of the real-time PCR. The proposed LAMP method can quickly and accurately detect prevalent FAdV via real-time turbidity assay, thereby providing a diagnostic platform for the prevention and control of the FAdV disease.
ISSN:1746-6148
1746-6148
DOI:10.1186/s12917-019-2015-5