Preconditioning with Substance P Restores Therapeutic Efficacy of Aged ADSC by Elevating TNFR2 and Paracrine Potential

Aging leads to a decline in stem cell activity by reducing the repopulation rate and paracrine potential, ultimately diminishing efficacy in vivo. TNF-α can exert inflammatory and cell death actions via Erk by binding to TNFR-1, and survival and tissue repair actions via Akt by binding to TNFR-2. Ag...

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Published in:Biology (Basel, Switzerland) Switzerland), 2023-11, Vol.12 (12), p.1458
Main Authors: Piao, Jiyuan, Cho, Hyunchan, Park, Jong Hyun, Yoo, Ki Hyun, Jeong, Ildoo, Hong, Hyun Sook
Format: Article
Language:English
Subjects:
adipose-derived stem cell
Aging
AKT protein
Antibiotics
Body fat
Bone marrow
Cell death
Cell proliferation
Cell survival
Cell therapy
Drug therapy
Ethylenediaminetetraacetic acid
Growth factors
Health aspects
Inflammation
Kinases
Paracrine signalling
Proteins
Recovery of function
Senescence
Stem cell transplantation
Stem cells
Substance P
Tissue engineering
TNF-a
TNFR2
Transplantation
Tumor necrosis factor receptors
Tumor necrosis factor-TNF
Tumor necrosis factor-α
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Summary:Aging leads to a decline in stem cell activity by reducing the repopulation rate and paracrine potential, ultimately diminishing efficacy in vivo. TNF-α can exert inflammatory and cell death actions via Erk by binding to TNFR-1, and survival and tissue repair actions via Akt by binding to TNFR-2. Aged cells are reported to have insufficient expression of TNFR-2, indicating that aged adipose-derived stem cells (ADSCs-E) lack the ability for cell survival and immune control compared to young ADSCs (ADSCs-Y). This study aims to assess the preconditioning effect of SP on the response of ADSCs-E to inflammation. ADSCs-E were treated with SP and then exposed to a high dose of TNF-α for 24 h. Consequently, ADSC-E exhibited weaker viability and lower TNFR2 levels compared to ADSC-Y. In response to TNF-α, the difference in TNFR2 expression became more pronounced in ADSC-E and ADSC-Y. Moreover, ADSC-E showed a severe deficiency in proliferation and paracrine activity. However, preconditioning with SP significantly enhanced the viability of ADSCs-E and also restored TNFR2 expression and paracrine potential, similar to ADSC-Y under inflammatory conditions. Our findings support the idea that preconditioning with SP has the potential to restore the cellular function of senescent stem cells before transplantation.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology12121458