Rapid and sensitive on-site detection of SARS-CoV-2 RNA from environmental surfaces using portable laboratory devices

This study presents the development of a highly sensitive on-site method for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA on various surfaces, including doorknobs and tables. Identifying SARS-CoV-2 RNA on these surfaces can be crucial in guiding decision-making for impl...

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Bibliographic Details
Published in:Microbiology spectrum 2023-12, Vol.11 (6), p.e0045623-e0045623
Main Authors: Kitamura, Kouichi, Ueno, Minami Kikuchi, Yoshida, Hiromu
Format: Article
Language:English
Subjects:
COVID-19 - diagnosis
enterovirus
Enterovirus Infections
Environmental Microbiology
environmental surface
Humans
Laboratories
Methods and Protocols
noroviruses
on-site detection
poliovirus
RNA, Viral - analysis
RNA, Viral - genetics
SARS-CoV-2
SARS-CoV-2 - genetics
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Summary:This study presents the development of a highly sensitive on-site method for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA on various surfaces, including doorknobs and tables. Identifying SARS-CoV-2 RNA on these surfaces can be crucial in guiding decision-making for implementing non-pharmaceutical interventions, such as zoning strategies, improving ventilation, maintaining physical distancing, and promoting increased hand hygiene practices. Moreover, the on-site detection system can facilitate the swift initiation of mitigation responses in non-laboratory settings, including long-term care facilities and schools. The protocols established in this study offer a comprehensive approach for achieving both sensitivity and rapidity in on-site SARS-CoV-2 RNA detection. Furthermore, since the RT-qPCR assay serves as the gold standard for detecting viral RNAs, the developed protocol holds potential for application to other viruses, including enteroviruses and noroviruses.
ISSN:2165-0497
2165-0497
DOI:10.1128/spectrum.00456-23