Pyrethroid susceptibility and oxidative detoxification mechanism in Colorado potato beetle and western corn rootworm

• Published in: Plant protection science 2023-01, Vol.59 (2), p.174-184
• Main Authors: Dworzańska, Daria, Zamojska, Joanna, Węgorek, Paweł, Bereś, Paweł K., Drzewiecki, Sławomir
• Format: Article
• Language: English
• Subjects: deltamethrin; diabrotica virgifera virgifera leconte; leptinotarsa decemlineata say; oxidative mechanism; piperonyl butoxide; susceptibility levels; tau-fluvalinate
• ISSN: 1212-2580; 1805-9341
• DOI: 10.17221/53/2022-PPS

The Colorado potato beetle (CPB) and the western corn rootworm (WCR) are among the world's most expansive insect pests. The aim of the study was to assess, for the first time in Poland, the levels of susceptibility to deltamethrin and tau-fluvalinate in the WCR and to determine the current status of susceptibility to those active substances in the CPB. In addition, the role of oxidative metabolism in the detoxification of pyrethroids was determined using piperonyl butoxide (PBO). The study found pyrethroid resistance in the CPB populations. The WCR populations exhibited "high susceptibility", "susceptibility", "low resistance" and "medium resistance" to the pyrethroids tested. Experiments using PBO showed that oxidative enzymes are involved in the deltamethrin detoxification mechanism in the CPB, although the effectiveness of this mechanism in Poland is decreasing. PBO was found to be highly toxic to the WCR populations. The findings from this study should be taken into account when developing a strategy to prevent the development of resistance in the pest species. The results for the toxicity of PBO to Polish WCR populations demonstrated that the enzymes inhibited by PBO are involved in important metabolic processes, which should be considered when developing innovative control methods for this pest.