Ex vivo pharmacokinetic/pharmacodynamic of hexahydrocolupulone against Clostridium perfringens in broiler chickens

• Published in: Frontiers in veterinary science 2024-05, Vol.11, p.1362292-1362292
• Main Authors: Zhang, Wanying, Lu, Yixing, Ma, Minglang, Yang, Jinyu, Huang, Huiguo, Peng, Xianfeng, Zeng, Zhenling, Zeng, Dongping
• Format: Article
• Language: English
• Subjects: broiler; Clostridium perfringens; ex vivo; hexahydrocolupulone; pharmacokinetic/pharmacodynamic (PK/PD); Veterinary Science
• ISSN: 2297-1769; 2297-1769
• DOI: 10.3389/fvets.2024.1362292

The economic impact of necrotizing enteritis (NE) resulting from infection has been significant within the broiler industry. This study primarily investigated the antibacterial efficacy of hexahydrocolupulone against , and its pharmacokinetics within the ileal contents of broiler chickens. Additionally, a dosing regimen was developed based on the pharmacokinetic/pharmacodynamic (PK/PD) model specific to broiler chickens. Results of the study indicated that the minimum inhibitory concentration (MIC) of hexahydrocolupulone against ranged from 2 mg/L to 16 mg/L in MH broth. However, in ileal content, the MIC ranged from 8 mg/L to 64 mg/L. The mutation prevention concentration (MPC) in the culture medium was found to be 128 mg/L. After oral administration of hexahydrocolupulone at a single dosage of 10-40 mg/kg bodyweight, the peak concentration ( ), maximum concentration time ( ), and area under the concentration-time curve (AUC) in ileal content of broiler chickens were 291.42-3519.50 μg/g, 1-1.5 h, and 478.99-3121.41 μg h/g, respectively. By integrating the PK and PD data, the AUC /MIC values required for achieving bacteriostatic, bactericidal, and bacterial eradication effects were determined to be 36.79, 52.67, and 62.71 h, respectively. A dosage regimen of 32.9 mg/kg at 24 h intervals for a duration of 3 days would yield therapeutic efficacy in broiler chickens against , provided that the MIC below 4 mg/L.