The role of HGF-MET pathway and CCDC66 cirRNA expression in EGFR resistance and epithelial-to-mesenchymal transition of lung adenocarcinoma cells

• Published in: Journal of hematology and oncology 2018-05, Vol.11 (1), p.74-74, Article 74
• Main Authors: Joseph, Nithila A, Chiou, Shiow-Her, Lung, Zoe, Yang, Cheng-Lin, Lin, Tze-Yi, Chang, Hui-Wen, Sun, H Sunny, Gupta, Sachin Kumar, Yen, Laising, Wang, Shulhn-Der, Chow, Kuan-Chih
• Format: Article
• Language: English
• Subjects: Acetylcholine receptors (nicotinic); Adenocarcinoma; Breast cancer; c-MET; c-Met protein; Cell adhesion & migration; Cell cycle; Cell growth; Chemotherapy; Circular RNA; Development and progression; Drug resistance; EGFR; EMT; Enzymes; Epidermal growth factor; Epidermal growth factor receptors; Epidermal growth factors; Gene expression; Genetic aspects; Hematology; Hepatocyte growth factor; HGF; Hypoxia; Immunocytochemistry; Immunohistochemistry; Kinases; Lung cancer; Mesenchyme; Metastases; Metastasis; MicroRNAs; Mutation; Oncology; Physiological aspects; Polymerase chain reaction; Post-translational modifications; Protein-tyrosine kinase; Proteins; Reverse transcription; Ribonucleic acid; RNA; SAE2; SUMO protein; SUMOylation; Western blotting
• ISSN: 1756-8722; 1756-8722
• DOI: 10.1186/s13045-018-0557-9

Epithelial-to-mesenchymal transition (EMT) has, in recent years, emerged as an important tumor cell behavior associated with high metastatic potential and drug resistance. Interestingly, protein SUMOylation and hepatocyte growth factor could respectively reduce the effect of small molecule inhibitors on tyrosine kinase activity of mutated epidermal growth factor receptor of lung adenocarcinomas (LADC). The actual mechanism is yet to be resolved. Immunohistochemistry was used to stain proteins in LADC specimens. Protein expression was confirmed by Western blotting. In vitro, expression of proteins was determined by Western blotting and immunocytochemistry. Levels of circular RNA were determined by reverse transcription-polymerase chain reaction. SAE2 and cirRNA CCDC66 were highly expressed in LADC. Expression of SAE2 was mainly regulated by EGFR; however, expression of cirRNA CCDC66 was positively regulated by FAK and c-Met but negatively modulated by nAchR7α. EGFR-resistant H1975 also highly expressed cirRNA CCDC66. Immediate response of hypoxia increased phosphorylated c-Met, SAE2, and epithelial-to-mesenchymal transition. Either activation of FAK or silencing of nAchR7α increased cirRNA CCDC66. HGF/c-Met regulates expression of SAE2 and cirRNA CCDC66 to increase EMT and drug resistance of LADC cells. Multimodality drugs concurrently aiming at these targets would probably provide more benefits for cancer patients.