COMPARISON OF BIOACTIVITY IN VITRO OF GLASS AND GLASS CERAMIC MATERIALS DURING SOAKING IN SBF AND DMEM MEDIUM

The surface reactivity of two sets of glasses and glass-ceramic materials belonging to the Li2O-SiO2-CaO-P2O5-CaF2 system were studied. The in-vitro bioactivity of coatings was evaluated using simulated body fluid (SBF) and Dulbecco's Modified Eagle's Medium (DMEM) soaking test in static r...

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Bibliographic Details
Published in:Ceramics (Praha) 2011-01, Vol.55 (3), p.199-207
Main Authors: Lutisanova, G, Palou, M T, Kozankova, J
Format: Article
Language:English
Subjects:
Biochemistry
Delay
DMEM medium
Fluorapatite
Glass
Glass ceramics
Glasses and glass ceramics
In vitro bioactivity
In vitro testing
Lithium disilicate
Phosphates
SBF
Soaking
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Summary:The surface reactivity of two sets of glasses and glass-ceramic materials belonging to the Li2O-SiO2-CaO-P2O5-CaF2 system were studied. The in-vitro bioactivity of coatings was evaluated using simulated body fluid (SBF) and Dulbecco's Modified Eagle's Medium (DMEM) soaking test in static regime for up to 28 days at 36.5 C in a microincubator. The changes in the surface structure were examined by SEM and EPMA. The functional groups of the silicate and phosphates were identified by IR. The crystal phases of the glasses and glass-ceramics were identified by XRD. The results suggest that all compositions of glasses as well as glass-ceramic samples after 28 days in the SBF and DMEM medium are bioactive. The surface characterisation and in-vitro tests revealed a few variations in the reactivity of the different glasses and glass-ceramic samples in their pristine form. The best results are seen for glass and glass-ceramic samples with a higher content of fluorapatite (FA). The use of the acellular culture medium DMEM resulted in a delay at the start of precipitation.
ISSN:0862-5468
1804-5847