Lung tissue biomechanics imaged with synchrotron phase contrast microtomography in live rats

The magnitude and distribution of strain imposed on the peripheral airspaces by mechanical ventilation at the microscopic level and the consequent deformations are unknown despite their importance for understanding the mechanisms occurring at the onset of ventilator-induced lung injury. Here a 4-Dim...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2022-03, Vol.12 (1), p.5056-1-5056-11, Article 5056
Main Authors: Cercos-Pita, Jose-Luis, Fardin, Luca, Leclerc, Hugo, Maury, Bertrand, Perchiazzi, Gaetano, Bravin, Alberto, Bayat, Sam
Format: Article
Language:English
Subjects:
631/443
631/443/1784
639/166
639/166/985
692/699/1785/3193
Alveoli
Animals
Biomechanical Phenomena
Biomechanics
Blood vessels
Humanities and Social Sciences
Life Sciences
Lung - diagnostic imaging
Lungs
Mechanical ventilation
Microscopy, Phase-Contrast
multidisciplinary
Pressure
Rats
Science
Science (multidisciplinary)
Synchrotrons
Ventilation
Ventilator-Induced Lung Injury
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The magnitude and distribution of strain imposed on the peripheral airspaces by mechanical ventilation at the microscopic level and the consequent deformations are unknown despite their importance for understanding the mechanisms occurring at the onset of ventilator-induced lung injury. Here a 4-Dimensional (3D + time) image acquisition and processing technique is developed to assess pulmonary acinar biomechanics at microscopic resolution. Synchrotron radiation phase contrast CT with an isotropic voxel size of 6 µm 3 is applied in live anesthetized rats under controlled mechanical ventilation. Video animations of regional acinar and vascular strain are acquired in vivo. Maps of strain distribution due to positive-pressure breaths and cardiovascular activity in lung acini and blood vessels are derived based on CT images. Regional strain within the lung peripheral airspaces takes average values of 0.09 ± 0.02. Fitting the expression S  =  kV n , to the changes in peripheral airspace area ( S ) and volume ( V ) during a positive pressure breath yields an exponent n = 0.82 ± 0.03, suggesting predominant alveolar expansion rather than ductal expansion or alveolar recruitment. We conclude that this methodology can be used to assess acinar conformational changes during positive pressure breaths in intact peripheral lung airspaces.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-09052-9