Locally invasive, castrate-resistant prostate cancer in a Pten/Trp53 double knockout mouse model of prostate cancer monitored with non-invasive bioluminescent imaging

Here we have improved an existing mouse model of prostate cancer based on prostate-specific deletion of Pten and Trp53 by incorporating a Cre-activatable luciferase reporter. By coupling the deletion of those genes to the activation of a luciferase reporter, we were able to monitor tumor burden non-...

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Bibliographic Details
Published in:PloS one 2020-09, Vol.15 (9), p.e0232807
Main Authors: Yong, Courtney, Moose, Devon L, Bannick, Nadine, Gutierrez, Wade R, Vanneste, Marion, Svensson, Robert, Breheny, Patrick, Brown, James A, Dodd, Rebecca D, Cohen, Michael B, Henry, Michael D
Format: Article
Language:English
Subjects:
Animals
Biology and Life Sciences
Bioluminescence
Biomarkers, Tumor - genetics
Carcinogenesis
Diagnosis
Disease Models, Animal
Engineering and Technology
Epithelial-Mesenchymal Transition
Genetic aspects
Health aspects
Luminescent Measurements
Male
Medicine and Health Sciences
Methods
Mice
Mice, Inbred C57BL
Mice, Knockout
Monitoring, Physiologic
Physiologic monitoring
Prostate cancer
Prostatic Neoplasms, Castration-Resistant - genetics
PTEN Phosphohydrolase - genetics
Research and Analysis Methods
Sequence Deletion
Tumor Suppressor Protein p53 - genetics
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Summary:Here we have improved an existing mouse model of prostate cancer based on prostate-specific deletion of Pten and Trp53 by incorporating a Cre-activatable luciferase reporter. By coupling the deletion of those genes to the activation of a luciferase reporter, we were able to monitor tumor burden non-invasively over time. We show that, consistent with previous reports, deletion of both Pten and Trp53 on a C57BL/6 background accelerates tumor growth and results in both the loss of androgen receptor expression and castrate resistant tumors as compared with loss of Pten alone. Loss of Trp53 results in the development of sarcomatoid histology and the expression of markers of epithelial-to-mesenchymal transition Zeb1 and vimentin, with kinetics and penetrance dependent on whether one or both alleles of Trp53 were deleted. Homozygous deletion of Trp53 and Pten resulted in uniformly lethal disease by 25 weeks. While we were able to detect locally invasive disease in the peritoneal cavity in aggressive tumors from the double knockout mice, we were unable to detect lymphatic or hematogenous metastatic disease in lymph nodes or at distant sites.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0232807