Integrating Bacteriophage onto the Magnetic Nanozyme for Effective Enrichment and Colorimetric Detection of Cronobacter sakazakii in Powdered Infant Formula

is a dangerous pathogen easily found in powdered infant formula (PIF), causing severe infections and even death in infants. Herein, a bacteriophage-immobilized magnetic nanozyme (Fe O @EspYZU13) was prepared for detection. Bacteriophage EspYZU13 isolated and identified by our group exhibits specific...

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Bibliographic Details
Published in:Foods 2024-11, Vol.13 (23), p.3788
Main Authors: Xu, Xuechao, Zhang, Yuansong, Gao, Lu, Yang, Juanli, Yang, Zhenquan
Format: Article
Language:English
Subjects:
Baby foods
Bacteria
bacterial enrichment
bacteriophage EspYZU13
colorimetric detection
Colorimetry
Cronobacter sakazakii
E coli
Enrichment
Enzymes
Health aspects
Hydrogen peroxide
Infant formulas
Infants
Iron oxides
magnetic nanozyme
Medical research
Nanomaterials
Peroxidase
Phages
powdered infant formula
Reagents
Staphylococcus infections
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Summary:is a dangerous pathogen easily found in powdered infant formula (PIF), causing severe infections and even death in infants. Herein, a bacteriophage-immobilized magnetic nanozyme (Fe O @EspYZU13) was prepared for detection. Bacteriophage EspYZU13 isolated and identified by our group exhibits specific lytic capacity. Fe O @EspYZU13 possesses remarkable enrichment capacity towards , efficiently enriching different concentrations of from a mixed bacterial solution. Furthermore, Fe O @EspYZU13 shows peroxidase-like activity, which can catalyze the 3,3',5,5'-tetramethylbenzidine (TMB) chromogenic reaction in the presence of H O . Upon introduction of , it can be specifically captured by Fe O @EspYZU13, inhibiting its peroxidase-like activity. Based on it, ranging from 3.2 × 10 to 3.2 × 10 CFU mL can be determined, offering a detection limit (LOD) of 26 CFU mL . Moreover, this reaction system keeps high specificity towards , which can resist interferences of other possible coexisting bacteria. in the artificially contaminated PIF can be detected, offering good recoveries (96.76% to 103.13%). These results indicate that our proposed reaction system demonstrates its practical application potential for efficient enrichment of from complex samples and accurate determination of in PIF.
ISSN:2304-8158
2304-8158
DOI:10.3390/foods13233788