Field evaluation of an iELISA and CF test for detection of IgG antibodies against Chlamydophila abortus in goats, sheep and rams

Blood sera samples from 99 clinically healthy goats, 230 ewes and 171 rams were investigated by complement fixation test (CFT) and indirect ELISA for the presence of IgG antibodies against Chlamydophila abortus. When comparing the use of CFT and iELISA, the seroprevalences were detected as follows:...

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Bibliographic Details
Published in:Veterinární medicína 2002-07, Vol.47 (7), p.195-198
Main Authors: Travnicek, M, Bhide, M.R. (Univerzita Veterinarskeho Lekarstva, Kosice (Slovak Republic)), Kovacova, D, Zubricky, P, Cislakova, L
Format: Article
Language:English
Subjects:
Antibodies
BELIER
BLOOD SERUM
BREBIS
CAPRIN
CAPRINOS
cf test
CHLAMYDIA PSITTACI
Chlamydophila abortus
Complement fixation
ELISA
EWES
GOATS
ielisa
Immunoglobulin G
IMMUNOGLOBULINE
IMMUNOGLOBULINS
INMUNOGLOBULINA
MORUECO
OVEJA
RAMS
Sensitivity analysis
Serology
SERUM SANGUIN
Sheep
SUERO SANGUINEO
TEST ELISA
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Summary:Blood sera samples from 99 clinically healthy goats, 230 ewes and 171 rams were investigated by complement fixation test (CFT) and indirect ELISA for the presence of IgG antibodies against Chlamydophila abortus. When comparing the use of CFT and iELISA, the seroprevalences were detected as follows: 3.03 and 24.24 % in goats, 3.04 and 11.3 % in ewes, 0 and 5.3 % in rams, respectively. Indirect ELISA was comparatively more sensitive than CFT in all three groups of animals. The iELISA may be used for screening at herd level like CFT, as there was moderate agreement (kappa=0.426) between these two tests.
ISSN:0375-8427
1805-9392
DOI:10.17221/5823-VETMED