Multigenerational Exposure to Uranium Changes Sperm Metabolome in Rats

Male infertility is a major public health issue that can be induced by a host of lifestyle risk factors such as environment, nutrition, smoking, stress, and endocrine disruptors. Regarding the human population exposed to uranium, it is necessary to explore these effects on male reproduction in multi...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-08, Vol.23 (15), p.8349
Main Authors: Grison, Stéphane, Legendre, Audrey, Svilar, Ljubica, Elie, Christelle, Kereselidze, Dimitri, Gloaguen, Céline, Lestaevel, Philippe, Martin, Jean-Charles, Souidi, Maâmar
Format: Article
Language:English
Subjects:
Animals
chronic exposure
Discriminant analysis
DNA methylation
Endocrine disruptors
Endocrine Disruptors - pharmacology
Exposure
Female
Humans
Infertility
Life Sciences
low-dose
Male
Mass spectrometry
Mass spectroscopy
Metabolites
Metabolome
metabolomic
Metabolomics
multigenerational
Nutrition
Pregnancy
Prenatal experience
Principal components analysis
Public health
Rats
Reproduction
Risk analysis
Risk factors
Semen
Sperm
Spermatozoa
Toxicology
Uranium
Uranium - toxicity
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Summary:Male infertility is a major public health issue that can be induced by a host of lifestyle risk factors such as environment, nutrition, smoking, stress, and endocrine disruptors. Regarding the human population exposed to uranium, it is necessary to explore these effects on male reproduction in multigenerational studies. The sensitivity of mass spectrometry (MS)-based methods has already proved to be extremely useful in metabolite identification in rats exposed to low doses of uranium, but also in human sperm. We applied this method to rat sperm over three generations (F0, F1 and F2) with multigenerational uranium exposure. Our results show a significant content of uranium in generation F0, and a reduction in the pregnancy rate only in generation F1. Based on principal component analysis (PCA), we observed discriminant profiles between generations. The partial least squares discriminant analysis (PLS-DA) of the 48 annotated variables confirmed that parental exposure of generation F0 (during both the preconceptional and prenatal periods) can have metabolic effects on spermatozoa for the next two generations. Metabolomics applied to epididymal spermatozoa is a novel approach to detecting the multigenerational effects of uranium in an experimental model, but could be also recommended to identify potential biomarkers evaluating the impact of uranium on sperm in exposed infertile men.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23158349