Phage and Antibiotic Combinations Reduce Staphylococcus aureus in Static and Dynamic Biofilms Grown on an Implant Material

causes the majority of implant-related infections. These infections present as biofilms, in which bacteria adhere to the surface of foreign materials and form robust communities that are resilient to the human immune system and antibiotic drugs. The heavy use of broad-spectrum antibiotics against th...

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Bibliographic Details
Published in:Viruses 2023-02, Vol.15 (2), p.460
Main Authors: Joo, Hyonoo, Wu, Sijia M, Soni, Isha, Wang-Crocker, Caroline, Matern, Tyson, Beck, James Peter, Loc-Carrillo, Catherine
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibacterial agents
Antibiotic resistance
Antibiotics
Bacteria
Bacterial infections
bacteriophage
Biocompatibility
biofilm
Biofilms
Biomedical materials
combination therapy
Dilution
Growth conditions
Humans
Immune system
Immunosuppressive agents
Microbiomes
Orthopedics
Phages
Staphylococcal Infections - drug therapy
Staphylococcus aureus
Staphylococcus infections
Staphylococcus Phages
Surgery
Transplants & implants
Vancomycin
Vancomycin - pharmacology
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Summary:causes the majority of implant-related infections. These infections present as biofilms, in which bacteria adhere to the surface of foreign materials and form robust communities that are resilient to the human immune system and antibiotic drugs. The heavy use of broad-spectrum antibiotics against these pathogens disturbs the host's microbiome and contributes to the growing problem of antibiotic-resistant infections. The use of bacteriophages as antibacterial agents is a potential alternative therapy. In this study, bioluminescent strains of S. aureus were grown to form 48-h biofilms on polyether ether ketone (PEEK), a material used to manufacture orthopaedic implants, in either static or dynamic growth conditions. Biofilms were treated with vancomycin, staphylococcal phage, or a combination of the two. We showed that vancomycin and staph phages were able to independently reduce the total bacterial load. Most phage-antibiotic combinations produced greater log reductions in surviving bacteria compared to single-agent treatments, suggesting antimicrobial synergism. In addition to demonstrating the efficacy of combining vancomycin and staph phage, our results demonstrate the importance of growth conditions in phage-antibiotic combination studies. Dynamic biofilms were found to have a substantial impact on apparent treatment efficacy, as they were more resilient to combination treatments than static biofilms.
ISSN:1999-4915
1999-4915
DOI:10.3390/v15020460