The Effect of Bifidobacterium Bifidum Supernatant and Cell Mass on the Proliferation Potential of Rat Bone Marrow-Derived Stromal Cells

Mesenchymal stem cells (MSCs) are widely used to treat various diseases, however, their proliferative potential reduces after a number of passages. It has been shown that some probiotics such as ( ) affect the proliferation of various cell lineages. The present study aimed to investigate the effect...

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Bibliographic Details
Published in:Iranian journal of medical sciences 2020-07, Vol.45 (4), p.269-276
Main Authors: Saberian, Mostafa, Shahidi Delshad, Elham, Habibi, Masoud
Format: Article
Language:English
Subjects:
Bacteria
bifidobacterium bifidum
Bone marrow
Cell growth
cell proliferation
EDTA
mesenchymal stromal cells
Original
Probiotics
Stem cells
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Summary:Mesenchymal stem cells (MSCs) are widely used to treat various diseases, however, their proliferative potential reduces after a number of passages. It has been shown that some probiotics such as ( ) affect the proliferation of various cell lineages. The present study aimed to investigate the effect of on the proliferation of rat bone marrow stromal cells (rBMSCs) and to develop a method for compensating their proliferation reduction after some passages. The present experimental study was conducted at Tehran University of Medical Sciences, Tehran, Iran, in 2017. The stromal cells were isolated from rBMSCs and their mesenchymal properties were confirmed by osteogenic and adipogenic differentiation media and staining. was cultured and the supernatant (BS) and bacterial cell mass (BCM) were extracted. The rBMSCs were treated with different concentrations of BS and BCM. The MTT assay was performed to measure the number of viable cells in the culture. Cell proliferation was analyzed using the paired-sample t test. Cell proliferation increased as the concentration of bacteria was increased logarithmically (0, 0.1, 0.3, 0.9, 3, 9, 30 μL/mL). In comparison with BS, cells treated with BCM showed increased cell proliferation at lower concentrations. This effect was caused by removing the "de Man, Rogosa, and Sharpe" (MRS) broth medium from the BCM culture. The optimal concentration of bacteria with the most significant effect on rBMSCs proliferation was determined. A significant increase in the proliferation of stromal cells was observed; confirming the stimulatory potential of probiotics ( ) on various cells. The use of products containing probiotic bacteria can increase the proliferation potential of BMSCs.
ISSN:0253-0716
1735-3688
DOI:10.30476/ijms.2019.45772