Tregs dysfunction aggravates postoperative cognitive impairment in aged mice

• Published in: Journal of neuroinflammation 2023-03, Vol.20 (1), p.75-75, Article 75
• Main Authors: Zhou, Yile, Ju, Huihui, Hu, Yan, Li, Tingting, Chen, Zhouyi, Si, Yuan, Sun, Xia, Shi, Yi, Fang, Hao
• Format: Article
• Language: English
• Subjects: Aging; Analysis; Animal cognition; Animals; Antibodies; Blood-brain barrier; Blood-Brain Barrier - metabolism; Brain; CD25 antigen; Cognition; Cognition disorders; Cognitive ability; Cognitive Dysfunction - etiology; Cognitive Dysfunction - metabolism; Complications; Complications and side effects; Cytokines; Cytokines - metabolism; Delirium - metabolism; Enzyme-linked immunosorbent assay; Epitopes; Flow cytometry; Hippocampus; Hippocampus - metabolism; Immune response; Immunofluorescence; Immunoregulation; Inflammation; Internal fixation in fractures; Laboratory animals; Lymphocytes T; Memory; Mice; Mice, Inbred C57BL; Nervous system; Neuroinflammation; Permeability; Plasma levels; Postoperative Cognitive Complications - etiology; Postoperative Cognitive Complications - metabolism; Postoperative cognitive dysfunction; Prevention; Proteins; Regulatory T cells; Risk factors; RNA sequencing; Signal transduction; Software; Statistical analysis; Surgery; T-Lymphocytes, Regulatory - pathology; Tibial Fractures - surgery; Tumor necrosis factor-α
• ISSN: 1742-2094; 1742-2094
• DOI: 10.1186/s12974-023-02760-7

Enhanced neuroinflammation is an important mechanism underlying perioperative neurocognitive disorders. Regulatory T cells (Tregs) play a crucial role in regulating systemic immune responses. The present study was aimed to investigate the participation of Tregs in the development of postoperative cognitive dysfunction (POCD). Surgery-associated neurocognitive disorder was induced in 18-month-old mice subjected to internal fixation of tibial fracture. Morris water maze was used to examine mice cognitive function. Splenic Tregs were collected for RNA sequencing and flow cytometry. Levels of inflammatory factors in the circulation and hippocampus were measured by enzyme-linked immunosorbent assay. Protein presences of tight junction proteins were detected by immunofluorescence. Surgery of internal fixation of tibial fracture induced cognitive impairment in aged mice, accompanied by elevated plasma levels of inflammatory factors and increased circulating Tregs. Transfusion of Tregs from young mice partially restored the structure of the blood-brain barrier and alleviated POCD in aged mice. Compared with young Tregs, differentially expressed genes in aged Tregs were enriched in tumor necrosis factor (TNF) signaling pathway and cytokine-cytokine receptor interaction. Flow cytometry revealed that aged Tregs had blunted functions under basal and stimulated conditions. Blockade of the CD25 epitope protected the blood-brain barrier structure, reduced TNF-α levels in the hippocampus, and improved surgery-associated cognition in aged mice. Blocking peripheral regulatory T cells improves surgery-induced cognitive function in aged mice. Therefore, aged Tregs play an essential role in the occurrence of POCD.