Effect of bacillus subtilis strain Z15 secondary metabolites on immune function in mice

• Published in: BMC genomics 2023-05, Vol.24 (1), p.273-273, Article 273
• Main Authors: Cao, Xi-Yuan, Aimaier, Reyihanguli, Yang, Jun, Yang, Jing, Chen, Zhong-Yi, Zhao, Jing-Jing, Yin, Li, Zhang, Qi, You, Jia, Zhang, Hui, Li, Hao-Ran, Chen, Jia-Yi, Mao, Qing-Chen, Yang, Li-Ping, Yu, Fei, Zhao, He-Ping, Zhao, Hui-Xin
• Format: Article
• Language: English
• Subjects: Abdomen; Analysis; Animals; Antifungal agents; Bacillus subtilis; Biological response modifiers; Blood; Care and treatment; Chromosome 1; Coagulation factors; Disease; Drugs; Encyclopedias; Fungicides; Gene expression; Genes; Genomes; Genomics; Health aspects; IgG antibody; Immune function; Immune response; Immune system; Immunoglobulin G; Immunoglobulin M; Immunoglobulins; Infection; Interferon; Interferon-gamma; Interleukin 6; Interleukins; Killer Cells, Natural - metabolism; Lymphocytes; Lymphocytes T; Macrophages; Metabolites; Mice; Molecular modelling; Monocytes; Mycoses; Natural killer cells; Phagocytosis; Physiological aspects; Physiology; Plant metabolites; Probiotics; Proteins; Resistance factors; Secondary metabolites; Spleen; T cell receptors; T cells; T-Lymphocytes - metabolism; Testing; Toll-like receptors; Transcriptome; Transcriptomes; Veins & arteries; γ-Interferon
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-023-09313-5

Previous studies have shown that secondary metabolites of Bacillus subtilis strain Z15 (BS-Z15) are effective in treating fungal infections in mice. To evaluate whether it also modulates immune function in mice to exert antifungal effects, we investigated the effect of BS-Z15 secondary metabolites on both the innate and adaptive immune functions of mice, and explored its molecular mechanism through blood transcriptome analysis. The study showed that BS-Z15 secondary metabolites increased the number of monocytes and platelets in the blood, improved natural killer (NK) cell activity and phagocytosis of monocytes-macrophages, increased the conversion rate of lymphocytes in the spleen, the number of T lymphocytes and the antibody production capacity of mice, and increased the levels of Interferon gamma (IFN-γ), Interleukin-6 (IL-6), Immunoglobulin G (IgG) and Immunoglobulin M (IgM) in plasma. The blood transcriptome analysis revealed 608 differentially expressed genes following treatment with BS-Z15 secondary metabolites, all of which were significantly enriched in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) terms for immune-related entries and pathways such as Tumor Necrosis Factor (TNF) and Toll-like receptor (TLR) signaling pathways, and upregulated expression levels of immune-related genes such as Complement 1q B chain (C1qb), Complement 4B (C4b), Tetracyclin Resistant (TCR) and Regulatory Factor X, 5 (RFX5). BS-Z15 secondary metabolites were shown to enhance innate and adaptive immune function in mice, laying a theoretical foundation for its development and application in the field of immunity.