KIF15 knockdown suppresses gallbladder cancer development

Gallbladder cancer (GBC) is commonly regarded as one of the most lethal malignant tumor types with poor prognosis. Kinesin family member 15 (KIF15) is reported to be tightly related with progression of multiple cancer types which, however, has not been clarified in GBC so far. KIF15 was significantl...

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Bibliographic Details
Published in:European journal of cell biology 2021-09, Vol.100 (7-8), p.151182-151182, Article 151182
Main Authors: Wang, Jun, Wang, Dandan, Fei, Zhewei, Feng, Dongxu, Zhang, Bo, Gao, Pingfa, Hu, Gangfeng, Li, Wenbing, Huang, Xia, Chen, Dawei, Ding, Xinde, Wu, Wei
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Cell Line, Tumor
Cell Proliferation
Gallbladder cancer
Gallbladder Neoplasms - genetics
Gene Expression Regulation, Neoplastic
Humans
KIF15
Kinesins
Mice
Migration
Molecular target
Phosphatidylinositol 3-Kinases - metabolism
Proliferation
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Summary:Gallbladder cancer (GBC) is commonly regarded as one of the most lethal malignant tumor types with poor prognosis. Kinesin family member 15 (KIF15) is reported to be tightly related with progression of multiple cancer types which, however, has not been clarified in GBC so far. KIF15 was significantly up-regulated in clinical GBC tissues compared with that in para-carcinoma tissues and the expression level was also correlated with tumor malignancies. In addition to tissues, GBC cells also exhibited a high expression abundance of KIF15. After down-regulating KIF15 via lentiviral transfection, GBC cell proliferation and migration were both inhibited, while cell apoptosis was promoted markedly. Likewise, silencing KIF15 significantly interfered the growth of nude mouse xenografts. Our experiments in GBC cell lines also demonstrated that KIF15 overexpression accelerated cell proliferation but lessened cell apoptosis in both GBC-SD and SGC-996 cells. Further investigation of the mechanism occurring in GBC inhibition mediated by KIF15 knockdown revealed that KIF15 deficiency led to decreased activity of several signaling pathways (TNF, PI3K/AKT and MAPK), a reduction of CDK6 expression regulated by enhanced p21, and HSP60 absence. Following the treatment of shCtrl- and shKIF15-transfected cells with AKT activator, we found that anti-tumor effects resulting from KIF15 deficiency could be relieved by AKT activator in both experimental cells. Overall, for the first time, we demonstrated that KIF15 was overexpressed in GBC and displayed a close relationship between KIF15 levels and GBC clinical stages. Furthermore, low expression of KIF15 resulted in obvious anti-tumor effects.
ISSN:0171-9335
1618-1298
DOI:10.1016/j.ejcb.2021.151182