Profiling Microglia From Alzheimer’s Disease Donors and Non-demented Elderly in Acute Human Postmortem Cortical Tissue

Microglia are the tissue-resident macrophages of the central nervous system (CNS). Recent studies based on bulk and single-cell RNA sequencing in mice indicate high relevance of microglia with respect to risk genes and neuro-inflammation in Alzheimer’s disease (AD). Here, we investigated microglia t...

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Published in:Frontiers in molecular neuroscience 2020-10, Vol.13, p.134-134
Main Authors: Alsema, Astrid M., Jiang, Qiong, Kracht, Laura, Gerrits, Emma, Dubbelaar, Marissa L., Miedema, Anneke, Brouwer, Nieske, Hol, Elly M., Middeldorp, Jinte, van Dijk, Roland, Woodbury, Maya, Wachter, Astrid, Xi, Simon, Möller, Thomas, Biber, Knut P., Kooistra, Susanne M., Boddeke, Erik W. G. M., Eggen, Bart J. L.
Format: Article
Language:English
Subjects:
Alzheimer's disease
barcoded Smart-seq2
Brain research
Central nervous system
Gene expression
Genomics
human
Macrophages
Microglia
Neurodegenerative diseases
Neuropathology
Neuroscience
Pathology
Ribonucleic acid
RNA
single-cell RNA sequencing
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Summary:Microglia are the tissue-resident macrophages of the central nervous system (CNS). Recent studies based on bulk and single-cell RNA sequencing in mice indicate high relevance of microglia with respect to risk genes and neuro-inflammation in Alzheimer’s disease (AD). Here, we investigated microglia transcriptomes at bulk and single-cell level in non-demented elderly and AD donors using acute human post-mortem cortical brain samples. We identified 7 human microglial subpopulations with heterogeneity in gene expression. Notably, gene expression profiles and subcluster composition of microglia did not differ between AD donors and non-demented elderly in bulk RNA sequencing nor in single-cell sequencing.
ISSN:1662-5099
1662-5099
DOI:10.3389/fnmol.2020.00134