Natural variations in a pectin acetylesterase gene, MdPAE10, contribute to prolonged apple fruit shelf life

Room‐temperature shelf life is a key factor in fresh market apple (Malus domestica Borkh.) quality and commercial value. To investigate the genetic and molecular mechanism underlying apple shelf life, quantitative trait loci (QTL) were identified using bulked segregant analysis via sequencing (BSA‐s...

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Bibliographic Details
Published in:The plant genome 2021-03, Vol.14 (1), p.e20084-n/a
Main Authors: Wu, Bei, Shen, Fei, Chen, Chi Jie, Liu, Li, Wang, Xuan, Zheng, Wen Yan, Deng, Yang, Wang, Ting, Huang, Zhen Yu, Xiao, Chen, Zhou, Qian, Wang, Yi, Wu, Ting, Xu, Xue Feng, Han, Zhen Hai, Zhang, Xin Zhong
Format: Article
Language:English
Subjects:
Acetylesterase
Chromosomes
Cultivars
Esterases
Ethylene
Fruit - genetics
Fruits
Genes
Genetic control
Malus - genetics
Malus domestica
Mutation
Pectin
Proteins
Quantitative Trait Loci
Shelf life
Single-nucleotide polymorphism
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Summary:Room‐temperature shelf life is a key factor in fresh market apple (Malus domestica Borkh.) quality and commercial value. To investigate the genetic and molecular mechanism underlying apple shelf life, quantitative trait loci (QTL) were identified using bulked segregant analysis via sequencing (BSA‐seq). Ethylene emission, flesh firmness, or crispness of apple fruit from 1,273 F1 plants of M. asiatica Nakai ‘Zisai Pearl’ × M. domestica ‘Golden Delicious’ were phenotyped prior to and during 6 wk of room‐temperature storage. Segregation of ethylene emission and the flesh firmness or crispness traits was detected in the population. Thirteen QTL, including three major ones, were identified on chromosome 03, 08, and 16. A candidate gene encoding pectin acetylesterase, MdPAE10, from the QTL Z16.1 negatively affected fruit shelf life. A 379‐bp deletion in the coding sequence of MdPAE10 disrupted its function. A single nucleotide polymorphism (SNP) in the MdPAE10 promoter region reduced its transcription activity. These findings provided insight into the genetic control of fruit shelf life and can be potentially used in apple marker‐assisted selection.
ISSN:1940-3372
1940-3372
DOI:10.1002/tpg2.20084