Cytomegalovirus infection in renal transplant recipients diagnosed by nested-PCR

A prospective study of cytomegalovirus (CMV) infection was carried out on 34 renal transplant recipients managed at a General Hospital in Ribeirão Preto, SP, Brazil. Serologic tests showed that all patients were infected with CMV before renal transplantation. Two nested-PCR techniques with primers t...

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Bibliographic Details
Published in:Brazilian journal of medical and biological research 2001-01, Vol.34 (1), p.93-101
Main Authors: Aquino, V H, Figueiredo, L T
Format: Article
Language:English
Subjects:
Antibodies, Viral - isolation & purification
Base Sequence
BIOLOGY
Brazil
Cytomegalovirus
Cytomegalovirus Infections - blood
Cytomegalovirus Infections - diagnosis
Cytomegalovirus Infections - urine
DNA Primers
glycoprotein B
glycoprotein H
Humans
Immunoglobulin G - isolation & purification
Immunoglobulin M - isolation & purification
Kidney Transplantation
MEDICINE, RESEARCH & EXPERIMENTAL
polymerase chain reaction
Polymerase Chain Reaction - methods
Prospective Studies
Viral Envelope Proteins - genetics
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Summary:A prospective study of cytomegalovirus (CMV) infection was carried out on 34 renal transplant recipients managed at a General Hospital in Ribeirão Preto, SP, Brazil. Serologic tests showed that all patients were infected with CMV before renal transplantation. Two nested-PCR techniques with primers that recognize sequences of the glycoprotein B (gB) and H (gH) genes were used for CMV detection in blood and urine samples during the post-transplantation period. CMV was detected more frequently in blood samples than in urine samples (P
ISSN:0100-879X
1414-431X
0100-879X
1414-431X
DOI:10.1590/S0100-879X2001000100011