Quantitative Determination of Stilbenoids and Dihydroisocoumarins in Shorea roxburghii and Evaluation of Their Hepatoprotective Activity

• Published in: International journal of molecular sciences 2017-02, Vol.18 (2), p.451-451
• Main Authors: Ninomiya, Kiyofumi, Chaipech, Saowanee, Kunikata, Yusuke, Yagi, Ryohei, Pongpiriyadacha, Yutana, Muraoka, Osamu, Morikawa, Toshio
• Format: Article
• Language: English
• Subjects: Animals; Bark; Cell activation; Cell Line; Chromatography, High Pressure Liquid; Cytotoxicity; D-Galactosamine; dihydroisocoumarin; Dipterocarpaceae - chemistry; Disease Models, Animal; Galactosamine - adverse effects; Hepatocytes; Hepatocytes - drug effects; Hepatocytes - metabolism; hepatoprotective effect; Humans; Isocoumarins - chemistry; Isocoumarins - pharmacology; Lipopolysaccharides; Lipopolysaccharides - adverse effects; Liver; Liver - drug effects; Liver - metabolism; Liver Diseases - drug therapy; Liver Diseases - etiology; Liver Diseases - metabolism; Liver Diseases - pathology; Macrophages; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - immunology; Macrophages, Peritoneal - metabolism; Male; mechanism of action; Mice; Nitric oxide; Nitric Oxide - metabolism; Peritoneum; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polyphenols; Polyphenols - chemistry; Polyphenols - pharmacology; Protective Agents - chemistry; Protective Agents - pharmacology; quantitative analysis; Resveratrol; Rodents; Shorea; Shorea roxburghii; Stilbenes - chemistry; Stilbenes - pharmacology; stilbenoid; Tumor Necrosis Factor-alpha - pharmacology; Tumor necrosis factor-TNF; Tumor necrosis factor-α
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms18020451

A simultaneous quantitative analytical method for 13 stilbenoids including (-)-hopeaphenol ( ), (+)-isohopeaphenol ( ), hemsleyanol D ( ), (-)-ampelopsin H ( ), vaticanols A ( ), E ( ), and G ( ), (+)-α-viniferin ( ), pauciflorol A ( ), hopeafuran ( ), (-)-balanocarpol ( ), (-)-ampelopsin A ( ), and -resveratrol 10- -β-d-glucopyranoside ( ), and two dihydroisocoumarins, phayomphenols A₁ ( ) and A₂ ( ) in the extract of (dipterocarpaceae) was developed. According to the established protocol, distributions of these 15 polyphenols ( - ) in the bark and wood parts of and a related plant were evaluated. In addition, the principal polyphenols ( , , , - ) exhibited hepatoprotective effects against d-galactosamine (d-galN)/lipopolysaccharide (LPS)-induced liver injury in mice at a dose of 100 or 200 mg/kg, p.o. To characterize the mechanisms of action, the isolates were examined in in vitro studies assessing their effects on (i) d-GalN-induced cytotoxicity in primary cultured mouse hepatocytes; (ii) LPS-induced nitric oxide (NO) production in mouse peritoneal macrophages; and (iii) tumor necrosis factor-α (TNF-α)-induced cytotoxicity in L929 cells. The mechanisms of action of these polyphenols ( , , and ) were suggested to be dependent on the inhibition of LPS-induced macrophage activation and reduction of sensitivity of hepatocytes to TNF-α. However, none of the isolates reduced the cytotoxicity caused by d-GalN.