Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of Dioscorea deltoidea , Tribulus terrestris and Panax japonicus on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus

• Published in: Nutrients 2021-10, Vol.13 (11), p.3811
• Main Authors: Povydysh, Maria N, Titova, Maria V, Ivanov, Igor M, Klushin, Andrey G, Kochkin, Dmitry V, Galishev, Boris A, Popova, Elena V, Ivkin, Dmitry Yu, Luzhanin, Vladimir G, Krasnova, Marina V, Demakova, Natalia V, Nosov, Alexander M
• Format: Article
• Language: English
• Subjects: adrenaline model; Animals; Biomass; Bioreactors; Blood; Blood Glucose - drug effects; Carbohydrate metabolism; Carbohydrate Metabolism - drug effects; Carbohydrates; Cardiovascular disease; Cell culture; Cell Culture Techniques; Cell suspensions; Cholesterol; Cholesterol - blood; Chromatography; Diabetes; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Type 2 - chemically induced; Diabetes Mellitus, Type 2 - drug therapy; Dioscorea; Dioscorea deltoidea; Disease prevention; Diuresis; Diuresis - drug effects; Epinephrine; Flowers & plants; furostanol-type glycosides; Ginsenosides; Glucose; Glycosides; Hematuria - drug therapy; Herbal medicine; Hyperglycemia; Laboratories; Laboratory animals; Lipid metabolism; Lipid Metabolism - drug effects; Lipids; Mass spectrometry; Medicinal plants; Metabolic disorders; Metabolites; NMR; Nuclear magnetic resonance; Panax; Physiology; Phytochemicals; plant cell culture; Plant Extracts - pharmacology; Plant species; Plants, Medicinal; Quantitative analysis; Quinic acid; Rats; Scientific imaging; Secondary metabolites; Streptozocin; streptozotocin-induced type 2 diabetes mellitus; Tribulus; Tribulus terrestris
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu13113811

In the present study, we explored the therapeutic potential of bioreactor-grown cell cultures of the medicinal plant species , and to treat carbohydrate metabolism disorders (CMDs) in laboratory rats. In the adrenaline model of hyperglycemia, aqueous suspensions of cell biomass pre-administered at a dose of 100 mg dry biomass/kg significantly reduced glucose level in animal blood 1-2.5 h ( and ) or 1 h ( ) after adrenaline hydrochloride administration. In a streptozotocin-induced model of type 2 diabetes mellitus, the cell biomass of and acted towards normalization of carbohydrate and lipid metabolism, as evidenced by a significant reduction of daily diuresis (by 39-57%), blood-glucose level (by 46-51%), blood content in urine (by 78-80%) and total cholesterol (25-36%) compared to animals without treatment. Bioactive secondary metabolites identified in the cell cultures and potentially responsible for their actions were deltoside, 25(S)-protodioscin and protodioscin in ; furostanol-type steroidal glycosides and quinic acid derivatives in ; and ginsenosides and malonyl-ginsenosides in These results evidenced for high potential of bioreactor-grown cell suspensions of these species for prevention and treatment of CMD, which requires further investigation.