Correlations Between LC-MS/MS-Detected Glycomics and NMR-Detected Metabolomics in Caenorhabditis elegans Development

This study examined the relationship between glycans, metabolites, and development in . Samples of N2 animals were synchronized and grown to five different time points ranging from L1 to a mixed population of adults, gravid adults, and offspring. Each time point was replicated seven times. The sampl...

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Bibliographic Details
Published in:Frontiers in molecular biosciences 2019-06, Vol.6, p.49-49
Main Authors: Sheikh, M Osman, Tayyari, Fariba, Zhang, Sicong, Judge, Michael T, Weatherly, D Brent, Ponce, Francesca V, Wells, Lance, Edison, Arthur S
Format: Article
Language:English
Subjects:
C. elegans
development
glycomics
mass spectrometry
metabolomics
Molecular Biosciences
NMR
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Summary:This study examined the relationship between glycans, metabolites, and development in . Samples of N2 animals were synchronized and grown to five different time points ranging from L1 to a mixed population of adults, gravid adults, and offspring. Each time point was replicated seven times. The samples were each assayed by a large particle flow cytometer (Biosorter) for size distribution data, LC-MS/MS for targeted - and -linked glycans, and NMR for metabolites. The same samples were utilized for all measurements, which allowed for statistical correlations between the data. A new protocol was developed to correlate Biosorter developmental data with LC-MS/MS data to obtain stage-specific information of glycans. From the five time points, four distinct sizes of worms were observed from the Biosorter distributions, ranging from the smallest corresponding to L1 to adult animals. A network model was constructed using the four binned sizes of worms as starting nodes and adding glycans and metabolites that had correlations with ≥ 0.5 to those nodes. The emerging structure of the network showed distinct patterns of - and -linked glycans that were consistent with previous studies. Furthermore, some metabolites that were correlated to these glycans and worm sizes showed interesting interactions. Of note, UDP-GlcNAc had strong positive correlations with many -glycans that were expressed in the largest animals. Similarly, phosphorylcholine correlated with many N-glycans that were expressed in L1 animals.
ISSN:2296-889X
2296-889X
DOI:10.3389/fmolb.2019.00049