Euonymus sachalinensis Induces Apoptosis by Inhibiting the Expression of c-Myc in Colon Cancer Cells

• Published in: Molecules (Basel, Switzerland) Switzerland), 2023-04, Vol.28 (8), p.3473
• Main Authors: Park, So-Mi, Jee, Wona, Park, Ye-Rin, Kim, Hyungsuk, Na, Yun-Cheol, Jung, Ji Hoon, Jang, Hyeung-Jin
• Format: Article
• Language: English
• Subjects: anti-cancer; Apoptosis; c-Myc; c-Myc protein; Cancer therapies; Caspase-3; Cell cycle; Cell death; Cell growth; Cell Line, Tumor; Cell Proliferation; Cell viability; Chemotherapy; Colon cancer; Colonic Neoplasms - metabolism; Colorectal cancer; Conjunctivitis; Deoxyribonucleic acid; DNA; Euonymus; Euonymus sachalinensis; Fluorouracil - pharmacology; Hay fever; Hormone replacement therapy; Humans; Lung cancer; Myc protein; Nephritis; oncogene; Protein expression; Proteins; Repressor Proteins; Rheumatoid arthritis; Rhinitis; Transcription factors
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28083473

We hypothesized that (ES) induces apoptosis by inhibiting the expression of c-Myc in colon cancer cells, and this study proved that the methanol extract of ES has anticancer effects in colon cancer cells. ES belongs to the Celastraceae family and is well known for its medicinal properties. Extracts of species belonging to this family have been used to treat diverse diseases, including rheumatoid arthritis, chronic nephritis, allergic conjunctivitis, rhinitis, and asthma. However, ES has been targeted because there are currently few studies on the efficacy of ES for various diseases, including cancer. ES lowers cell viability in colon cancer cells and reduces the expression of c-Myc protein. We confirm that the protein level of apoptotic factors such as PARP and Caspase 3 decrease when ES is treated with Western blot, and confirm that DNA fragments occur through TUNEL assay. In addition, it is confirmed that the protein level of oncogenes CNOT2 and MID1IP1 decrease when ES is treated. We have also found that ES enhances the chemo-sensitivity of 5-FU in 5-FU-resistant cells. Therefore, we confirm that ES has anticancer effects by inducing apoptotic cell death and regulating the oncogenes CNOT2 and MID1IP1, suggesting its potential for use in the treatment of colon cancer.