Changes on the Caco-2 secretome through differentiation analyzed by 2-D differential in-gel electrophoresis (DIGE)

• Published in: International journal of molecular sciences 2012-11, Vol.13 (11), p.14401-14420
• Main Authors: García-Lorenzo, Andrés, Rodríguez-Piñeiro, Ana M, Rodríguez-Berrocal, Francisco J, Cadena, María Páez de la, Martínez-Zorzano, Vicenta S
• Format: Article
• Language: English
• Subjects: Biomarkers; Biomarkers - metabolism; Caco-2; Caco-2 Cells; Cells; Colorectal cancer; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; differentiation; DIGE; Genotype & phenotype; Humans; Neoplasm Grading; Proteins; Proteome - metabolism; Proteomics - methods; secretome; Signal transduction; Two-Dimensional Difference Gel Electrophoresis
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms131114401

Colorectal cancer is still a major health burden worldwide, and its diagnosis has not improved in recent years due to a lack of appropriate diagnostic serum markers. Aiming to find new diagnostic proteins, we applied the proteomic DIGE technology to analyze changes in the secretome before/after differentiation of the colon adenocarcinoma Caco-2 cell line, an accepted in vitro model to study colorectal tumorigenesis. When the secretomes from undifferentiated (tumor-like) and differentiated cells (resembling healthy enterocytes) were compared, we found 96 spots differentially expressed. After MS/MS analysis, 22 spots corresponding to 15 different proteins were identified. Principal component analysis demonstrated these 22 spots could serve as a discriminatory panel between the tumor-like and normal-like cells. Among the identified proteins, the translationally-controlled tumor protein (TCTP), the transforming growth factor-beta-induced protein ig-h3 (TGFβIp), and the Niemann-Pick disease type C2 protein (NPC2) are interesting candidates for future studies focused on their utility as serum biomarkers of colorectal cancer.