Proteomic analysis reveals dynamic changes in cloacal fluid composition during the reproductive season in a sexually promiscuous passerine

Cryptic female choice (CFC) is a component of postcopulatory sexual selection that allows females to influence the fertilization success of sperm from different males. While its precise mechanisms remain unclear, they may involve the influence of the protein composition of the female reproductive fl...

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Published in:Scientific reports 2024-06, Vol.14 (1), p.14259-10, Article 14259
Main Authors: Míčková, Kristýna, Jelínek, Václav, Tomášek, Oldřich, Stopková, Romana, Stopka, Pavel, Albrecht, Tomáš
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Language:English
Subjects:
631/181/2470
631/443/494
631/601
Animals
Cloaca - metabolism
Egg laying
Female
Females
Fertilization
Humanities and Social Sciences
Male
multidisciplinary
Passeriformes - metabolism
Passeriformes - physiology
Protein composition
Proteins
Proteome - analysis
Proteome - metabolism
Proteomes
Proteomics
Proteomics - methods
Reproduction - physiology
Reproductive cycle
Reproductive system
Science
Science (multidisciplinary)
Seasons
Sexual Behavior, Animal - physiology
Sexual selection
Sperm
Spermatozoa - metabolism
Spermatozoa - physiology
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creator Míčková, Kristýna
Jelínek, Václav
Tomášek, Oldřich
Stopková, Romana
Stopka, Pavel
Albrecht, Tomáš
description Cryptic female choice (CFC) is a component of postcopulatory sexual selection that allows females to influence the fertilization success of sperm from different males. While its precise mechanisms remain unclear, they may involve the influence of the protein composition of the female reproductive fluids on sperm functionality. This study maps the protein composition of the cloacal fluid across different phases of female reproductive cycle in a sexually promiscuous passerine, the barn swallow. Similar to mammals, the protein composition in the female reproductive tract differed between receptive (when females copulate) and nonreceptive phases. With the change in the protein background, the enriched gene ontology terms also shifted. Within the receptive phase, distinctions were observed between proteomes sampled just before and during egg laying. However, three proteins exhibited increased abundance during the entire receptive phase compared to nonreceptive phases. These proteins are candidates in cryptic female choice, as all of them can influence the functionality of sperm or sperm-egg interaction. Our study demonstrates dynamic changes in the cloacal environment throughout the avian breeding cycle, emphasizing the importance of considering these fluctuations in studies of cryptic female choice.
doi_str_mv 10.1038/s41598-024-62244-3
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subjects 631/181/2470
631/443/494
631/601
Animals
Cloaca - metabolism
Egg laying
Female
Females
Fertilization
Humanities and Social Sciences
Male
multidisciplinary
Passeriformes - metabolism
Passeriformes - physiology
Protein composition
Proteins
Proteome - analysis
Proteome - metabolism
Proteomes
Proteomics
Proteomics - methods
Reproduction - physiology
Reproductive cycle
Reproductive system
Science
Science (multidisciplinary)
Seasons
Sexual Behavior, Animal - physiology
Sexual selection
Sperm
Spermatozoa - metabolism
Spermatozoa - physiology
title Proteomic analysis reveals dynamic changes in cloacal fluid composition during the reproductive season in a sexually promiscuous passerine
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