Loop-mediated isothermal amplification for detection of Legionella pneumophila in respiratory specimens of hospitalized patients in Ahvaz, southwest Iran

Legionnaires' disease is an important public health problem that can cause substantial mortality and morbidity. Legionnaires' disease-risk estimation may be compromised by uncertainties in -detection methods. The aim of this study was the detection of in respiratory specimens of hospitaliz...

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Bibliographic Details
Published in:Infection and drug resistance 2019-03, Vol.12, p.529-534
Main Authors: Moosavian, Mojtaba, Seyed-Mohammadi, Sakineh, Saki, Morteza, Shahi, Fatemeh, Khoshkholgh Sima, Mahtab, Afshar, Davoud, Barati, Sara
Format: Article
Language:English
Subjects:
Alveoli
Antigens
Bacterial infections
Bronchus
Charcoal
Communicable diseases
Deoxyribonucleic acid
Diagnosis
Diseases
DNA
EDTA
Epidemiology
Health aspects
Hospital patients
Identification
Infections
Infectious diseases
Iran
Isolation
Laboratories
LAMP
Legionella
Legionella pneumophila
Legionnaires' disease
Lung diseases
Medical schools
Methods
MIP gene
Morbidity
Mortality
Original Research
PCR
Pneumonia
Public health
Public health movements
Respiratory symptoms
Sputum
Teachers
Urine
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Summary:Legionnaires' disease is an important public health problem that can cause substantial mortality and morbidity. Legionnaires' disease-risk estimation may be compromised by uncertainties in -detection methods. The aim of this study was the detection of in respiratory specimens of hospitalized patients with respiratory symptoms by culture, PCR, and loop-mediated isothermal amplification (LAMP) methods. Sputum and bronchoalveolar lavage samples were obtained from patients with pneumonia admitted to teaching hospitals in Ahvaz, Iran from June 2016 to March 2017. Isolation of spp. was done by culturing the samples directly onto buffered charcoal-yeast extract and modified Wadowsky-Yee agar medium. Then, PCR and LAMP assays were performed for detection of via its gene in respiratory specimens. A total of 100 respiratory specimens were collected. Our results showed that 1% of the samples were culture positive for spp., and 3% and 7% of samples were positive for using the gene on PCR and LAMP assays, respectively. Legionnaires' disease should be considered in the diagnosis of pulmonary infectious diseases. Also, the LAMP assay is a faster method with higher sensitivity and specificity than conventional methods, such as PCR and culture, for laboratory diagnosis of Legionnaires' disease.
ISSN:1178-6973
1178-6973
DOI:10.2147/IDR.S198099