Leaves from banana (Musa nana) and maize (Zea mays) have no phyto-prophylactic effects on the susceptibility of grass carp (Ctenopharyngodon idella) to Aeromonas hydrophila infection

• Published in: BMC veterinary research 2017-11, Vol.13 (1), p.329-8, Article 329
• Main Authors: Mayrhofer, Richard, Menanteau-Ledouble, Simon, Pucher, Johannes, Focken, Ulfert, El-Matbouli, Mansour
• Format: Article
• Language: English
• Subjects: Aeromonas hydrophila; Animal Feed; Animals; Carps - growth & development; Carps - microbiology; Diet composition; Dietary Supplements; Disease Susceptibility - veterinary; Feed conversion ratio; Fish Diseases - diet therapy; Fish Diseases - microbiology; Fisheries; Gram-Negative Bacterial Infections - diet therapy; Gram-Negative Bacterial Infections - microbiology; Gram-Negative Bacterial Infections - veterinary; Haematocrit; Musa; Phytogenic supplement; Plant Leaves; Stable isotope δ13C; Stress indicator; Zea mays
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-017-1255-5

The ubiquitous and opportunistic bacterial pathogen Aeromonas hydrophila has been associated with ulcerative dermatitis in fish, especially under stressful conditions. It can cause severe losses in fresh water aquaculture and is particularly prevalent in tropical and subtropical regions. Fresh leaves from maize and bananas have been used as feed supplement by fish farmers in Vietnam and it has been reported that they may have phyto-prophylactic benefits. In the present study, a feeding trial was conducted to investigate the benefits of providing maize and banana leaves as feed supplement: to determine if they were taken up and digested by grass carp (Ctenopharyngodon idella), if this uptake resulted in improved growth performance, and if leaf supplementation protected fish when challenged with A. hydrophila by intramuscular injection. All fish were fed an identical ratio of commercial pelleted feed relative to biomass. However, in 12/18 tanks, this diet was supplemented with either fresh banana leaves or fresh maize leaves; offered ad libitum. Addition of leaves increased the overall feed conversion ratio (FCR) significantly. However, if only the pellet were taken into account, then no difference was found between treatments. Changes to the isotopic composition of the fish showed leaf nutrient uptake occurred. No prophylactic effects of feeding banana or maize leaves were detected against infection with A. hydrophila, and the diet did not induce changes in the fish haematocrit. However, addition of the maize leaves was associated with significantly reduced severity of the skin lesions, which could improve the market value of the fish. Addition of the leaf supplement did not result in significantly improved growth performance. Similarly, the effect of the supplement on the fish survival to infection was not significant.