Reduced metabolic capacity in fast and slow skeletal muscle via oxidative stress and the energy‐sensing of AMPK/SIRT1 in malnutrition

• Published in: Physiological reports 2021-03, Vol.9 (5), p.e14763-n/a
• Main Authors: Hirabayashi, Takumi, Nakanishi, Ryosuke, Tanaka, Minoru, Nisa, Badur un, Maeshige, Noriaki, Kondo, Hiroyo, Fujino, Hidemi
• Format: Article
• Language: English
• Subjects: AMP-Activated Protein Kinases - metabolism; Animals; Atrophy; Body weight; Body weight loss; Citrate synthase; Diet; Energy; Enzymes; Food intake; Intolerance; Kinases; Laboratory animals; Low protein diet; Male; Malnutrition; metabolic capacity; Metabolism; Metabolites; Methods; Mitochondria; Mitochondria - metabolism; Mitochondrial DNA; Muscle Fibers, Fast-Twitch - metabolism; Muscle Fibers, Slow-Twitch - metabolism; Muscle, Skeletal - metabolism; Muscular Atrophy - metabolism; Musculoskeletal system; Nutrient deficiency; Original; Oxidative stress; Oxidative Stress - physiology; Physiology; Plasma; Proteins; Rats; Rats, Wistar; SIRT1; SIRT1 protein; Sirtuin 1 - metabolism; Skeletal muscle; Soleus muscle; Stress analysis; Succinate dehydrogenase; Transcription Factors - metabolism
• ISSN: 2051-817X
• DOI: 10.14814/phy2.14763

The effects of malnutrition on skeletal muscle result in not only the loss of muscle mass but also fatigue intolerance. It remains unknown whether the metabolic capacity is related to the fiber type composition of skeletal muscle under malnourished condition although malnutrition resulted in preferential atrophy in fast muscle. The purpose of the present study was to investigate the effects of metabolic capacity in fast and slow muscles via the energy‐sensing of AMPK and SIRT1 in malnutrition. Wistar rats were randomly divided into control and malnutrition groups. The rats in the malnutrition group were provided with a low‐protein diet, and daily food intake was limited to 50% for 12 weeks. Malnutrition with hypoalbuminemia decreased the body weight and induced the loss of plantaris muscle mass, but there was little change in the soleus muscle. An increase in the superoxide level in the plasma and a decrease in SOD‐2 protein expression in both muscles were observed in the malnutrition group. In addition, the expression level of AMPK in the malnutrition group increased in both muscles. Conversely, the expression level of SIRT1 decreased in both muscles of the malnutrition group. In addition, malnutrition resulted in a decrease in the expression levels of PGC‐1α and PINK protein, and induced a decrease in the levels of two key mitochondrial enzymes (succinate dehydrogenase and citrate synthase) and COX IV protein expression in both muscles. These results indicate that malnutrition impaired the metabolic capacity in both fast and slow muscles via AMPK‐independent SIRT1 inhibition induced by increased oxidative stress. Malnutrition impaired mitochondrial metabolic capacity via downregulating SIRT1 induced by increased oxidative stress in both fast and slow muscles although no atrophy was observed in slow muscle. The energy‐sensing in malnutrition was characterized by AMPK‐independent SIRT1 inhibition in both fast and slow muscles.