A Medicago truncatula Cell Biology Resource: Transgenic Lines Expressing Fluorescent Protein-Based Markers of Membranes, Organelles, and Subcellular Compartments

Ivanov discuss their study on Medicago truncatula which is used widely for studies of two major plant-microbe symbioses, the N2-fixing association with rhizobia and the endosymbiosis with arbuscular mycorrhizal fungi or studies of legume interactions with pathogens and pests, as well as legume biolo...

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Bibliographic Details
Published in:Molecular plant-microbe interactions 2023-04, Vol.36 (4), p.256-259
Main Authors: Ivanov, Sergey, Daniels, Dierdra A, Harrison, Maria J
Format: Article
Language:English
Subjects:
actin
Alfalfa
apoplast
Arbuscular mycorrhizas
Biology
Cell Membrane - metabolism
Cytoskeleton
Endoplasmic Reticulum
Fluorescence
Genetic transformation
Golgi Apparatus - metabolism
Golgi Apparatus - ultrastructure
Golgi bodies
Legumes
mCherry
Medicago truncatula
Medicago truncatula - genetics
Membranes
microtubules
Nitrogen fixation
Organelles
Pests
Phosphatidylinositol 4,5-diphosphate
Phosphatidylinositol 4-phosphate
Phosphoinositides
Plant cells
Proteins
Symbiosis
Transgenic plants
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Summary:Ivanov discuss their study on Medicago truncatula which is used widely for studies of two major plant-microbe symbioses, the N2-fixing association with rhizobia and the endosymbiosis with arbuscular mycorrhizal fungi or studies of legume interactions with pathogens and pests, as well as legume biology in general. The 18 transgenic M. truncatula lines constitutively express fluorescent protein markers of membranes, organelles, cytoskeletal elements, and two phosphoinositides, namely, phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and phosphatidylinositol 4-phosphate (PI4P), in cells throughout the plant. M. truncatula transgenic lines expressing mCherry-based markers under the control of the constitutive AtUBQ10 promoter were generated by Agrobacterium tumefaciens--mediated transformation of M. truncatula R108.
ISSN:0894-0282
1943-7706
DOI:10.1094/MPMI-01-22-0023-A